And a US intelligence counterpart to DARPA is planning to fund research into detecting organisms
containing gene drives and other modifications.
Next week, the Intelligence Advanced Research Projects Agency (IARPA), which is part of the Office of the US Director of National Intelligence, will hold a meeting about a planned funding programme for detecting genetically modified organisms that are potentially harmful, including ones that
contain gene drives.
When creating the antimalaria antibody gene drive, the researchers had to inject Cas9, guide RNAs and bits of DNA
containing the gene drive into the egg.
Not exact matches
A few days after Chalfie's call, Prasher
drove to the post office with a little tube
containing the GFP
gene, put it in a padded envelope, and mailed it to Columbia.
When the researchers bred female flies
containing the yellow
gene drive to normal males, 95 to 100 percent of both male and female progeny were yellow.
The chunks of DNA that broke off all
contained genes that help
drive cancer progression, and they moved to prime locations in the genome where they would be most active.
Several more projects explicitly focus on
gene drive applications: A group at UC Riverside led by molecular biologist Omar Akbari will try to document the genetic diversity of the Aedes aegypti mosquito and test ways to limit or reverse
gene drives in
contained test environments.
pAAV - SEPT - Acceptor, the preferred vector,
contains a promoterless splice acceptor - IRES - neoR
gene, which enhances the efficiency of homologous integration via
gene trap enrichment, whereas pAAV - TK - Acceptor has a conventional heterologous promoter -
driven TK - neoR
gene.
A self - inactivating lentiviral construct
containing a mouse phosphoglycerate kinase - 1 internal promoter
driving the human
gene encoding GDNF [44] was used to generate GDNF - secreting hNPCctx.
HV cells constitutively expressing the LacZ
gene were generated by electroporation with a vector
containing a CAG
driven β - Geo cDNA followed by selection in G418 (150 µg / ml) for 2 wk.