They added these beads to the sample to be tested, and then added that mixture to a test tube
containing magnetic beads coated with DNA strands that bind the other half of the DNA.
Following the provided protocol, a portion of the RNA -
containing lysate (5 — 10 µl) was hybridized at 54 degrees C overnight to RNA specific
magnetic capture
beads in the presence of blocking buffers, proteinase K and preordered mRNA probe sets specific for the genes of interest: HMOX1, Nrf2, Keap1, Bach1, and GAPDH.