Sentences with phrase «containing supernatants»

Not exact matches

The group has over 10 years» experience with this methodology, with specific expertise in cytokine / chemokine detection in tissue culture supernatants and plasma samples with panels containing up to 42 analytes.

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

Briefly, the appropriate dilution of tissue supernatant was added to the cuvette containing 1.9 mL of 50 mmol phosphate buffer (pH 7.0).

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

1 ml of antibody - containing hybridoma supernatant is only $ 40, significantly cheaper than commercially available antibodies.

Immunoglobulin - containing HB1.F3.H2IgG NSC culture supernatant was subjected to purification by protein A affinity chromatography.

In addition, we showed that, although the supernatants from each of the HCMV - infected cultures contained infectious virus, the viral copy number and the quantity and timing of virus production varied among the various organ - specific MSC.

The supernatant, the medium in which the culture is grown, contains a multitude of beneficial byproducts of the growth process, including vitamins, enzymes, antioxidants, and immune stimulators.