In addition to the control chow, some of the APP / PS1 mice were fed three experimental chows enriched with fish oil and having a similar fat content as the control chow: fish oil supplement only, plant sterol supplement or Fortasyn supplement, which
contains uridine - monophosphate, phospholipids, B - vitamins, and antioxidants.
All of the cells were allowed to attach for 4 hr before the elongation - device chamber was filled with complete medium
containing the mitotic inhibitors [mitotic inhibitor 1 (MI1), 5 μm cytosine β - d - arabinofuranoside (AraC; Sigma, St. Louis, MO), 20 μm 5 - fluoro - 2 ′ - deoxyuridine (5FdU; Sigma), and 20 μm
uridine (Sigma)-RSB-.