We have previously shown that during anterograde heart
contractions hemolymph is expelled from the dorsal vessel through an excurrent opening located near the head (Figure 1A)[3].
Similarly, during retrograde heart
contractions hemolymph is expelled from the dorsal vessel through an excurrent opening located in the 8th abdominal segment (Figure 1A)[3].
Not exact matches
For the simultaneous visualization of
hemolymph flow through the heart and ventral abdominal
contractions, as well as the quantification of
hemolymph flow speeds in the lateral abdomen, mosquitoes were restrained laterally by placing pins: (1) on either side of the cervical membrane such that they crossed dorsally and restrained the mosquito between the pins and the Sylgard; (2) through non-vascular portions of each wing after they had been teased away from the abdominal tergum; (3) between the wings and the anterior portion of the abdomen; (4) over the legs, entering the Sylgard posterior to the third leg pair; and (5) immediately dorsal of the longitudinal midline of the scutum (Figure 1B).
However, in general, the periodic discharge of
hemolymph from the heart and into the posterior abdomen during periods of retrograde heart
contractions results in net anterograde
hemolymph flow in the extracardiac abdominal hemocoel (Videos S2 and S3).
The strong, exclusive correlation between abdominal and anterograde heart
contractions suggests that abdominal
contractions play more than an accidental role in insect
hemolymph propulsion and are essential for proper
hemolymph circulation.
Specifically, the abdomen contracts in a retrograde peristaltic manner exclusively during anterograde heart
contraction periods, resulting in increased extracardiac
hemolymph propulsion in a dorsoretrograde direction.