Sentences with phrase «control cultures precultured»

Acute insulin stimulation increased glucose transport by 21 % in control cultures precultured at 1 pmol / l insulin, but at higher insulin concentrations we could not detect an effect of insulin on glucose uptake.

Not exact matches

In accordance with other studies, we found an increased glucose uptake under acute insulin stimulation in cultures established from control subjects precultured under basal physiological insulin concentrations, but when precultured at a higher insulin concentration, we could not see an effect on acute insulin stimulation indicative of induced insulin resistance (10).

Preculturing myotubes for 4 days at increasing insulin concentrations did not change the basal FV0.1 in diabetic or control cultures (Figs. 3A and B).

We evaluated the capacity for glucose uptake in control and diabetic cultures precultured under three different insulin concentrations and found that when precultured with 1 pmol / l and 1 nmol / l insulin, the basal glucose uptake was reduced by 30 % (P < 0.05) in diabetic cultures compared with control cultures (Fig. 5).