Sentences with phrase «coronal brain sections»
Frozen coronal brain sections (10 µm thick) were fixed in 4 % paraformaldehyde in phosphate buffer (PB; pH 7.4) overnight at 4 °C, treated with proteinase K (10 µg / ml) for 15 min and then with HCl (0.2 N) for 10 min, followed by acetic - anhydride solution for 10 min at room temperature.
http://journals.plos.org/
(C — N) Expression analysis of Acks by in situ hybridization using coronal brain sections of seizure - induced (Sz - induced)(C — H) or control Japanese honeybee workers (I — N).
Not exact matches
Brains were fixed with 4 % paraformaldehyde in 100 mM sodium tetraborate, pH 9.5, for 3 h, cryoprotected with 20 % sucrose - potassium - PBS (KPBS), and sectioned into coronal (30 μm) sections using a sliding microtome (Leica Microsystems Inc, Buffalo Grove, IL, USA).
(F) Immunohistochemical analysis was done using brain coronal sections from these same mice with the antibody 6E10.
The complete mouse brain profile is represented by serial coronal sections of adult mouse brain, 16 µm thick.
Brains were cut into 30 µm coronal sections on a sliding microtome (Leica) and the free - floating sections were used for immunostaining.
FoxP2 expression in area X of multiple coronal or sagittal brain sections was quantified from digitized images of film autoradiograms using Adobe Photoshop 7.0 (Adobe Systems Inc..