Sentences with word «coverslip»

Sections were mounted with glass coverslips and images were taken using a microscope (Axioplan; Zeiss) connected to a CCD camera (RT slider; Diagnostic Instruments).

At 24 hours post fertilization (hpf), fluorescent embryos were dechorionated, mounted on coverslips in 1.2 % ultra-low gelling agarose, overlaid with 30 % Danieua / PTU and analyzed by laser scanning confocal microscopy (Zeiss LSM510 Meta).

Basically, the veterinarian will mix a fresh fecal sample from the dog with a special solution, strain the mixture, put it into a test tube covered with a glass lid, spin it down in a centrifuge and then look at the material that sticks to the underside of the glass coverslip under a microscope.

The result is more sensitive imaging at twice the speed, all for the cost of an aluminum - coated coverslip.

Coverslips plated with acutely dissociated hNPCctx - GDNF or hNPCctx were fixed with 4 % paraformaldehyde, washed with PBS, blocked in 5 % normal donkey serum and 0.1 % Triton X-100, and incubated with goat anti-GDNF (1 ∶ 100; R&D Systems) primary antibody followed by donkey anti-goat Cy3 - conjugated secondary antibody (1 ∶ 1000; Jackson IR).

Finally, the researchers experimented with performing diSPIM with a mirrored coverslip.

Its unique ibidi Polymer Coverslip Bottom guarantees superior optics for high - end microscopy.

Horizontal specimen mounting supports conventional preparations such as coverslips and Petri dishes and provides a platform for custom - designed chambers.

The preps were grown for 14 days in culture and plated onto coverslips.

To prepare slides, PBS (20 μL) was added to the surface of each microscope slide and then the removed coverslips were inverted and placed on the PBS.

«Serial,» De la Mora's first solo show in Europe, debuts engrossing abstract works that are visually minimal but hold conceptual weight, due to the artist's exacting process and the unsuspecting found materials he employs — including discarded parts from printing presses and microscope coverslips.

HCT - 116 cells grown on coverslips were treated with Bleomycin (20 - 80µM) for 4 hrs followed by 2 hrs of recovery.

Bulk - cultured PC3 - GFP cells (A), holoclone P1H8 (B)-- , or meroclone P2H3 (C)-- derived cells were plated on glass coverslips and stained for CD44, α2β1, and / or β - catenin using monoclonal antibodies.

Cells were resuspended in HEPES buffer containing the following (in mm): 136 NaCl, 5.6 KCl, 1 MgCl2, 2.2 CaCl2, 11 glucose, 10 HEPES; pH adjusted to 7.4 with NaOH before plating on poly - d - lysine (0.02 mg / ml; BD Bioscience) and laminin (0.02 mg / ml; Sigma - Aldrich)- coated coverslips.

The samples are being preserved in a cedar wood oil and are sealed with a coverslip to prevent contamination.

In another version they call triple - view SPIM, they added an extra objective below the coverslip, to collect extra output and improve spatial resolution at no cost to speed, and with no added dose of light.

Instead, they preferred to put the worm embryos on standard glass coverslips.

At one time, coverslips, mirrors and lenses ruled the world of microscopy.

«The main motivation of this new research was that the resolution in diSPIM was limited by the numerical aperture of the upper lenses, and fluorescence emitted in the direction of the coverslip is not captured,» explained Hari Shroff, leader of the research team.

This is important for researchers because, as Shroff said, «Life did not evolve on a coverslip.»

Following injection a coverslip was mounted over the embryos ready for microscopy.

Embryos were dechorionated in bleach and mounted on a coverslip under Voltalef oil as previously described [57].

Fura -2-AM (50 μm, Invitrogen) stock was prepared in HEPES buffer containing 2.5 % DMSO and 0.01 % Pluronic F - 127 (Invitrogen) and then pipetted into the medium bathing the coverslips to a final concentration of 2 μm Fura -2-Am.

Chambers were 500 μm wide, 100 μm high and 7 mm long, and were constructed from poly (dimethylsiloxane) bonded to glass coverslips.

The chambers were fixed to the stage of an inverted spinning disk confocal microscope (Nikon, Andor), which was used to capture images of the cell populations residing on the coverslip glass.

The cultures were then incubated at room temperature for one hour to allow cells to attach to the glass coverslip.

If a coverslip with cells is accidentally dropped and your sample side up and orientation is lost, you can still continue the experiment by carefully picking up the coverslip with tweezers: gently scrape one surface of the coverslip with a pipette tip to see if any cells are visibly removed to define coverslip orientation before mounting your sample.

Coverslips were counterstained with 4, 6 - diamidino -2-phenylindole (DAPI), mounted, sealed and observed.

Please note: If a coverslip with cells is accidentally dropped and your sample side up and orientation is lost, you can still continue the experiment by carefully picking up the coverslip with tweezers: gently scrape one surface of the coverslip with a pipette tip to see if any cells are visibly removed to define coverslip orientation before mounting your sample.

Some mounting media solutions have DAPI already added and will harden after exposure to air, eliminating the need to seal the edges of the coverslip.

These mixtures were examined microscopically (magnification × 400) on a slide overlaid with a coverslip.

Coverslips were mounted with ProLong Gold anti-fade reagent with DAPI (Invitrogen).

Apply an appropriate fluorophore - conjugated secondary antibody diluted in antibody dilution buffer to the coverslips and incubate for 1 hour in a moist, dark environment.

Cells were plated either on glass coverslips (40,000 cells / coverslip) coated with poly - L lysine and laminin or six - well plates (106 cells / well) coated with laminin alone.

At least five fields from each of three coverslips were photographed with a Nikon E600 equipped with epifluorescence, using SPOTcam and SPOT advanced software (Diagnostic Instruments, Inc.).

Nuclei were counterstained with Hoechst 33258 (1 ∶ 10,000; Sigma) and coverslips were mounted in GelTol Aqueous mounting media (Immunotech).

Sections were then counterstained in Mayer's haematoxylin and mounted under coverslips.

TIRF only excites fluorophores within a few hundred nanometers of the coverslip (4).

Coverslips (22 × 22 mm; VWR, Radnor, PA) were placed on the slides and sealed with rubber cement (Staples, Framingham, MA).

Following hybridization, coverslips were removed, and slides were washed in 0.4 × SSC / 0.3 % NP - 40 (Abbott Molecular, Inc.) at 73 °C for 2 min and then in 2 × SSC / 0.1 % NP - 40 (Abbott Molecular, Inc.) for 1 min at room temperature.

Bulk - cultured PC3 or PC3 - GFP cells, or clone - derived cultures were plated on glass coverslips and used in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).

The samples were preserved in two drops of Vectashield Mounting Medium containing 1.5 μg / ml of 4» 6 - diamidino -2-phenylindole (DAPI; Vector Laboratories, Burlingame, CA), and covered with a coverslip.

Coverslips were then sealed using nail polish and dried in the dark for 10 min.

Transfer the scraped material to a slide, and place a coverslip to enhance sample examination and avoid contamination of the microscope objective with mineral oil.