Enter HITS - CLIP, a new approach that applies high throughput sequencing of RNAs isolated by
crosslinking immunoprecipitation.
Called individual nucleotide resolution
crosslinking immunoprecipitation (iCLIP), the technique enabled Sanford's lab to identify IGF2BP3 binding sites in several hundred RNA transcripts in two B - ALL cell lines.
Not exact matches
«fCLIP - seq stands for formaldehyde
crosslinking,
immunoprecipitation, and sequencing.
We will map the mRNA interactome of DDX6 and mutant variants using protein - RNA
crosslinking,
immunoprecipitation, and next - generation sequencing to identify mRNAs where DDX6 binds, the region within the mRNA, and how the repertoire of putative targets changes.