The method employs cryo - electron microscopy to diffract extremely small 3 - D protein crystals, a million to 100 million times smaller in volume (100 times per side) than used in a traditional X-ray
crystallography experiment.
The Purdue team mimicked the conditions of the serial femtosecond
crystallography experiment, but used electron paramagnetic resonance to reveal the electronic configurations of the molecules, Pushkar said.
Involvement with other tasks: Protein crystallization will be carried out at UROM and preparation for Serial femtosecond
crystallography experiments.
Dress worn by Lawrence Bragg's wife Alice, design based on the patterns created through Lawrence's
crystallography experiments
Not exact matches
The creation of XFEL facilities, including the Linac Coherent Light Source (LCLS) and the European X-FEL, have created opportunities for conducting new
experiments which can overcome the limitations of traditional
crystallography.
This technique, which scientists are rapidly automating, may eventually displace
crystallography as the tool of choice in many cell biology studies because it does not require the subject to crystallize and sometimes can even reveal multiple conformational states in a single
experiment.
At that time, the
crystallography field was becoming increasingly automated, to the point that the
experiments themselves becoming remotely controllable.
Many protein structures are elucidated to atomic detail using x-ray
crystallography or nuclear magnetic resonance (NMR)
experiments and are deposited in the PROTEIN DATA BANK (PDB).
Since his departure from the Lab, Chapman has continued with his interest in molecular imaging with FELs and in 2009 led an international collaboration to carry out the first
experiments in this area at LCLS, where a global team introduced a new technique now called serial femtosecond
crystallography (SFX).