Sentences with phrase «culture conditions»
Culture conditions refer to the environment or setup in which something grows or develops, particularly when it comes to living organisms. It includes factors such as temperature, nutrients, humidity, and other conditions necessary for the proper growth and survival of organisms in a specific culture or artificial setting. Full definition
The right culture conditions for different bird species require a lot of development to get right.
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However, we could not find differences between different culture conditions of the two groups.
Their method relied on briefly bathing blood cells from newborn mice in a mildly acidic solution and then tweaking culture conditions to produce stem cells.
The right culture conditions for different bird species require a lot of development to get right.
«In the future, this will move us towards a way to understand how to generate a cardiac muscle or other specific cell type without having to try an unwieldy number of cell culture conditions.»
Isolated endothelial progenitor cells (EPCs) were expanded in vitro under low cell density culture conditions (Figure 1A: 103 cells / cm2).
«This research enables us to get one step closer to the optimal culture conditions for muscle stem cells.»
That's an important lesson, Tolar says; it suggests that future attempts to correct genetic skin diseases should focus on culture conditions that nourish these stem cells, and potentially even target them for modification.
Bartosz Balana (Ravens, TUD)-- «Influence of culture conditions on differentiation and bioelectrical properties of human mesenchymal stem cells» (2005)
A team headed by Prof Dr Julia Bandow and Christoph Senges from the research group Applied Microbiology at Ruhr - Universität Bochum analysed a broad spectrum of the bacterium's metabolic products under various culture conditions.
We have recently optimized culture conditions for human satellite cells (1) and shown that cultures established from healthy subjects express GS activity to the same level as seen in in vivo biopsies (2).
Siva V comments, «This work, primarily driven by Massimo Nichane and team, demonstrated that a single respiratory cell type — Sox9 + progenitors of lung — can be used to regenerate the lungs and can be propagated in vitro using defined culture conditions.
Progenitor cells with a fluorescent green marker were isolated from mice, and culture conditions which preserved the marker, meaning that the cells were maintaining their function, were assessed.
Skeletal muscle stem cells propagated as myospheres display electrophysiological properties modulated by culture conditions.
The study demonstrates that HFF cells are very useful tools for iPS cell derivation and culture condition optimization.
«We also think this method could be applied towards the development of culture conditions during differentiation of human stem cells.
Several growth factors, cytokines, and chemicals have been used in muscle stem cell cultures, but the optimal culture conditions required to maintain the undifferentiated state, inhibit differentiation, and enhance eventual transplantation efficiency have not yet been established.
«It will be exciting to explore how cell culture conditions enhance the zygote's intrinsic surveillance and repair mechanisms, thus leading to better quality embryos and potentially more successful pregnancies.»
In 2009, from experiments at the RIKEN Center for Developmental Biology in Kobe, Japan, he found that when culture conditions are right, adding a single ingredient — bone morphogenetic protein 4 (Bmp4)-- with precise timing is enough to convert embryonic cells to PGCs.
Molecular Devices integrates this type of software into high - throughput screening platforms, so scientists can quickly scan thousands of spheroids growing in varied culture conditions.
The availability of powerful assays and convenient culture conditions make the fruit fly system ideally suited for the planned laboratory research.
«We found that the more complex, dynamic culturing conditions (like those used in this project) are necessary to accurately predict renal drug toxicity in human systems,» said Sakolish.
Instead of such static culture conditions, Dawson and her colleagues turned to a dual flow microfluidic chamber — which removes wastes and supplies nutrients via continuous liquid streams — and can keep tissues viable for prolonged periods of time.
Using novel culture conditions Dr. Reimer's team has shown it is now possible to maintain the cells for 16 days in vitro.
«We isolated kidney progenitor cells from mice and searched for the most favorable culture conditions,» said Assistant Professor Shunsuke Tanigawa of Kumamoto University.
They then placed the cells into culture conditions containing certain cytokines, and instead of differentiating into B - cells, the cells continued to divide as stem cells.
Johns Hopkins stem cell research John Gearhart, who did not participate in the study, says the findings strengthen the case for making more lines available to researchers because it shows that «changes are occurring even under ideal [cell] culture conditions.»
Phase - contrast images of organ - derived endothelial cells (37 °C culture conditions) that had undergone two successive selection processes by flow cytometry.
From the eggs provided by Grouse Park, Crystal Biosciences researchers were able to establish five viable cell lines from a series of experimental culture conditions.
Their data show that hESC grown on laminin - 521 show a decreased proneness to acquiring DNA damage during suboptimal culture conditions, such as medium acidification during high culture density.
And then ESC researchers can stop worrying about laws, regulations, and court orders and get back to worrying about culture conditions, tumors, cell differentiation, and all the other scientific puzzles that remain to be solved.
Please refer to the Cellartis DEF ‑ CS 500 Culture System User Manual and the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System User Manual for specific culture conditions and protocols.
This ensures high karyotype stability over long periods and hence reproducible culture conditions.
In this study, we have shown evidence that skeletal muscle cells established from type 2 diabetic subjects, grown and fused under basal physiological culture conditions, retain defects in insulin - stimulated GS activity, indicating that the insulin - resistant phenotype is intrinsic to the cells.
To highlight the potentially important role of 3D culture conditions, the authors compared 2D monolayers of hepatocyte progenitors, fetal liver stromal cells, or adult bone marrow - derived stromal progenitor cells to their 3D counterparts cultured on liver extracellular matrix (3D - ECM) constructs.
Stem cell methodologies like culture conditions, sorting, and how to grow and transfect cells are all tools we can apply now to human brain cancers.
In light of the observation that iPS cell derivation takes place under the same culture conditions used for ES cells [20], we hypothesized that these human feeder cells could offer a stable tool for defining molecular hallmarks during conversion of differentiated somatic cells to the pluripotent state.
This could reflect intrinsic differences between mouse and human NSCs, but might still be attainable by altering some experimental parameters such as infecting more target cells, extending culturing conditions, and incubating target cells with a rho - kinase inhibitor small molecule, shown to enhance survival of human ES cells [30], [31].
«The model will help scientists identify new approaches to design and establish cell culture models for other human cells that previously had resisted viable culture conditions,» said Ozdener
Importantly, cisplatin toxicity and Pgp efflux transporter activity measured on - chip more closely mimic the in vivo responses than results obtained with cells maintained under conventional culture conditions.