Sentences with phrase «culture conditions for»

We demonstrate the utility of this method using an example in which the culture conditions for the microbial production of a bioactive human protein are optimized.

The current findings could provide important clues to determine the culture conditions for promoting the differentiation of primate ES cells into mature gametes, and to understand molecular mechanisms of primate gametogenesis including the timing of germ cell induction, the regulation of germ cell gene expression, and the response to growth factors for germ cell differentiation.

Cells were cultured under normal culture conditions for 24 h and the supernatants were subjected to PGE2 ELISA.

In order to improve culture conditions for maturation of cumulus - free oocytes in vitro, we began by assaying the expression of 15 growth factors (BDNF, IGF - I, estradiol, GDNF, leptin, FGF1, FGF2, GM - CSF, EGF, TGF - α, TGF - β1; 2; 3, and ET - 1; 2) and 27 of their cognate receptors that have been shown to regulate oocyte maturation.

The right culture conditions for different bird species require a lot of development to get right.

The second step will be to optimize culture conditions for the commitment of the iPS / hES cell - derived retinal precursors towards the photoreceptor lineage.

«This research enables us to get one step closer to the optimal culture conditions for muscle stem cells.»

Not exact matches

Like Sachs, Whippman believes that «there are many reasons why life in America is likely to produce anxiety compared with other developed nations: long working hours without paid vacation time for many, insecure employment conditions with little legal protection for workers, inequality, and the lack of universal health care coverage, to name a few,» but she stresses that our «happiness - seeking culture» is also part of the problem.

For example, under what conditions does the working culture accept innovation and failure?

[16:00] Pain + reflection = progress [16:30] Creating a meritocracy to draw the best out of everybody [18:30] How to raise your probability of being right [18:50] Why we are conditioned to need to be right [19:30] The neuroscience factor [19:50] The habitual and environmental factor [20:20] How to get to the other side [21:20] Great collective decision - making [21:50] The 5 things you need to be successful [21:55] Create audacious goals [22:15] Why you need problems [22:25] Diagnose the problems to determine the root causes [22:50] Determine the design for what you will do about the root causes [23:00] Decide to work with people who are strong where you are weak [23:15] Push through to results [23:20] The loop of success [24:15] Ray's new instinctual approach to failure [24:40] Tony's ritual after every event [25:30] The review that changed Ray's outlook on leadership [27:30] Creating new policies based on fairness and truth [28:00] What people are missing about Ray's culture [29:30] Creating meaningful work and meaningful relationships [30:15] The importance of radical honesty [30:50] Thoughtful disagreement [32:10] Why it was the relationships that changed Ray's life [33:10] Ray's biggest weakness and how he overcame it [34:30] The jungle metaphor [36:00] The dot collector — deciding what to listen to [40:15] The wanting of meritocratic decision - making [41:40] How to see bubbles and busts [42:40] Productivity [43:00] Where we are in the cycle [43:40] What the Fed will do [44:05] We are late in the long - term debt cycle [44:30] Long - term debt is going to be squeezing us [45:00] We have 2 economies [45:30] This year is very similar to 1937 [46:10] The top tenth of the top 1 % of wealth = bottom 90 % combined [46:25] How this creates populism [47:00] The economy for the bottom 60 % isn't growing [48:20] If you look at averages, the country is in a bind [49:10] What are the overarching principles that bind us together?

CEO Jean - Laurent Bonnafé observed that in a challenging environment for the entire banking industry, BNP Paribas succeeded in maintaining its client franchise, profitability and strong risk - management culture, while adapting the organization to changing market conditions and regulation.

By my reckoning, the social conditions that made him so alluring — most importantly the nostalgia for an integral Christian culture — are no longer in place.

By my reading of both the human condition and our current culture, a project like Hart's is more important to the status of religion in public life than, say, arguments for a natural law.

An educational program aiming to nurture citizens who can function justly within the mosaic of American culture and within the world's multicultural pluralism should teach students the dynamics of various hegemonic orders, the reasons for their emergence, the conditions of their continuence, the factors that lead to their decline and fall.

What is less clear to me is why complementarians like Keller insist that that 1 Timothy 2:12 is a part of biblical womanhood, but Acts 2 is not; why the presence of twelve male disciples implies restrictions on female leadership, but the presence of the apostle Junia is inconsequential; why the Greco - Roman household codes represent God's ideal familial structure for husbands and wives, but not for slaves and masters; why the apostle Paul's instructions to Timothy about Ephesian women teaching in the church are universally applicable, but his instructions to Corinthian women regarding head coverings are culturally conditioned (even though Paul uses the same line of argumentation — appealing the creation narrative — to support both); why the poetry of Proverbs 31 is often applied prescriptively and other poetry is not; why Abraham, Isaac, and Jacob represent the supremecy of male leadership while Deborah and Huldah and Miriam are mere exceptions to the rule; why «wives submit to your husbands» carries more weight than «submit one to another»; why the laws of the Old Testament are treated as irrelevant in one moment, but important enough to display in public courthouses and schools the next; why a feminist reading of the text represents a capitulation to culture but a reading that turns an ancient Near Eastern text into an apologetic for the post-Industrial Revolution nuclear family is not; why the curse of Genesis 3 has the final word on gender relationships rather than the new creation that began at the resurrection.

And this means that, as a quite concrete historical condition, the only choice that remains for the children of post-Christian culture is not whom to serve, but whether to serve Him whom Christ has revealed or to serve nothing — the nothing.

It is that tradition that underlies the fruitless quest of Hirst and Peters for necessary and sufficient conditions, as well as the Platonically inspired educational theories of Bloom and Hirsch which assume that we can delineate «higher» levels of knowledge and culture that must be transmitted to the young.

- God, the Absolute - humanity, the human condition in its universal characteristics, - male and female, though different, equal in rights and dignity, - the cosmos, especially the planet earth available, with its limited resources, for all humanity - the planet's ecology as common essential source of life and hence of concern for all humans, present and future, - the human conscience guiding each one interiorly would be known only to each one personally, - the each group of humans has a history and a religio - cultural background of its own is a universal factor that makes for particularity and different contexts for theology, - the realization that the present increasing globalization of relationships, economy and culture impinge on theology and spirituality universally, though differently.

In the Christian Institute for the Study - of Religion and Society there was an open discussion about a proposal that since Christ transcended not only cultures but also religions and ideologies, the fellowship of confessors of faith in Jesus as the Messiah should not separate from their original religious or secular ideological community but should form fellowships of Christian faith in those communities themselves, and that so long as the Law sees baptism as transference from one community to another it should not be made the condition of entry into the fellowship of the sacrament of the Lord's Supper but made a sacramental privilege for a later time (Ref.

It is all the more imperative, then, that ample provision also be made for studies that are not subordinate to nonacademic interests, in order that the prevailing conditions of culture and society may not remain without challenge or alternatives.

John Paul II wrote in the apostolic exhortation Christifideles Laici: «The common outcry, which is justly made on behalf of human rights — for example, the right to health, to home, to work, to family, to culture — is false and illusory if the right to life, the most basic and fundamental right and the condition of all other personal rights, is notdefended with maximum determination.»

Within the changing conditions and evolving life on this planet, and out of the various developing cultures that have shaped us, we humans can and do create meaning for ourselves.

It means that the culture of this and other nations rather than the reality of Christendom is becoming the conditioning frame of reference for the Protestant church.

You must be mistaken... why, the conservatives HAD to be at the forefront of assuring the rights of freed slaves, ending the deliberate government policy of destroying native cultures, extending the vote to ex-slaves and women, ending child labor, creating safer and better working conditions for laborers, etc?

Speaking during Premier's News Hour, she said: «We have whole issues with purity culture and modesty culture which says, «well, women, if you dress a certain way it's your fault if a man behaves in that way» and so actually for a lot of women they have been conditioned to blame themselves and say «if he's done that to me it must be my fault».

But at the same time, a culture of life can not be achieved without some element of righteous anger — at the injustices that create the conditions for abortion, at the abortionists who perpetrate it, and at the organizations which profit from it.

Given the conditions prevailing in our culture, we have the best and most effective way ever devised for gathering large and prosperous congregations.

A genuine Christian interiority must provide the inspiration for a humanism capable of living a vigorous, free and open life within one's culture, whatever its condition may be.

The determined character of the synthesis insures that any novelty which is actualized will be integrally connected with real conditions in the world and provides the continuity necessary for generalizations to be used in science, technology, and culture.

Besides the conditions of society itself, under which family and friends had primary responsibility for the care of the dying and the dead, memento mon were spread throughout culture: in the church's art, in morality plays like Everyman, in drinking songs, in the ordinary artifacts of everyday life (e.g., in Austria a towel hanger portraying a human form split down the middle: one half a beautiful young woman, the other a skeleton) To be sure, the specter of death (and judgment) has been used as a form of social control.

The increase of the number of Americans with non-Protestant orientations created for the first time a condition in which Protestantism's domination of American culture could be challenged.

But as Postman has pointed out, in a culture thoroughly conditioned by electronic media, the main problems for imaginatively imparting information in educational institutions are radically different from what Whitehead had in mind or probably could have suspected.

What's coming is an administration in which the U.S. Agency for International Development, which dispenses billions of dollars of foreign aid, will continue to condition that aid on recipient nations» bowing to the LGBT agenda — thus making enemies for America in traditional societies and cultures that will learn to equate «democracy» with libertine bullying.

This appeared to us a refusal to acknowledge the extent of Christ's human condition, even while it offered chilling rationale for the culture of abortion.

Their stance for chastity and monogamy, often labeled as «unrealistic,» is actually much better suited to African culture, especially in its present troubled condition, than are modern Western teachings about sexuality emphasizing personal freedom and individual development.

The PDF, Microbes Make the Cheese, A Report from the American Academy for Microbiology explains: Cheese is created by orderly successions of microbial communities that produce compounds responsible for cheese flavor... Each piece of cheese contains as many as 10,000,000,000 or 10 billion microbes... The added starter cultures dominate the cheese microbiota, establishing conditions that select for the next microorganisms that will be capable of thriving in the changing cheese matrix.

«APAC is a region in constant evolution with a growing cocktail culture and high demand for quality, premium spirits and these are ideal conditions for the gins to grow.»

Culturing can be a bit of a fussy thing until you find the conditions that work best for you.

Joe Milner, Milestone Beverages» managing director who previously worked with Diageo, Constellation and Quintessential, commented: «APAC is a region in constant evolution with a growing cocktail culture and high demand for quality, premium spirits and these are ideal conditions for the gins to grow.

It therefore may be better to allow young athletes to participate strength and conditioning training as nod to the culture and to satisfy a perceived psychological need for more training and focusing instead on making sure that the training is limited and age - and developmentally - appropriate.

It was great for them to see how other cultures get around besides in an air conditioned van.

We describe here how the western dismantling of three fundamental evolved maternal nighttime infant caregiving practices, specifically, how to lay your baby down to sleep, how to feed your baby, and where to locate your baby for sleep, created the conditions that favored and fostered the emergence within western industrialized cultures of SIDS.

For thousands of years, it has been used in many cultures worldwide as an antiseptic, antibacterial treatment on all kinds of skin and health conditions.

As well as giving you a chance to see how the knowledge and skills you are acquiring can be applied, you will also see the conditions under which scientists work and get a feel for workplace culture — all providing valuable insight for the career decisions you'll be making in your final year.

To study the role of sweet taste receptors, Cohen and lead study author Robert J. Lee, PhD, a post doctoral researcher in his lab, partnered with colleagues from the Children's Hospital of Philadelphia and the Monell Chemical Senses Center to analyze cultures and tissue samples from the upper respiratory tract of patients who were undergoing sinonasal surgery for a variety of conditions.

The urgency to find treatments for life - threatening conditions translates into a company culture that is fast - paced, hardworking, and entrepreneurial in spirit, says Clare Carmichael, chief human resources officer for Alexion.

In 2009, from experiments at the RIKEN Center for Developmental Biology in Kobe, Japan, he found that when culture conditions are right, adding a single ingredient — bone morphogenetic protein 4 (Bmp4)-- with precise timing is enough to convert embryonic cells to PGCs.

The availability of powerful assays and convenient culture conditions make the fruit fly system ideally suited for the planned laboratory research.

Using novel culture conditions Dr. Reimer's team has shown it is now possible to maintain the cells for 16 days in vitro.

«For decades, researchers have tried to generate human pancreatic beta cells that could be cultured and passaged long term under conditions where they produce insulin.