Sentences with phrase «culture dishes with»

The colonies were fixed in culture dishes with 4 % paraformaldehyde in phosphate - buffered saline (0.01 M PBS, pH 7.4) for 20 min at room temperature (RT) followed by washing with PBS (2 × 5 min).

The cells were enzymatically removed from the culture dish with Tryple ™ Select (Invitrogen) for 15 min at 37 °C, resuspended in 1 ml FACS buffer I (2 % FBS, 0.01 % sodium azide in PBS), and counted with Neubauer cell counter chambers.

Not exact matches

Because it's a heck of a lot easier to dish it out than it is to eat it up, let me tell you, and I think sometimes we inadvertently perpetuate celebrity culture by railing so loudly against it, by feeding into the caricatures with our derision.

Smørrebrød — an open - faced rye sandwich piled high with toppings — is one of the most essential dishes in the Danish culture, most commonly eaten for...

I have used Ellie Krieger's recipe with a few tweaks on quantity (I increased the garlic and I added lemon zest and red pepper flakes) since I love her simplistic approach to the dish which borrows from the Israeli (the use of tahini) and other Levantine cultures (parsley, garlic, and lemon).

I recently posted a recipe for a Hungarian dish that uses onions, peppers and tomatoes and did make note that probably every culture with access to those ingredients has a similar dish — and there you go — proving the point.

Filed Under: paleo diet, Snacks & Sides Tagged With: food culture, french fries, Gluten - free, Grain - free, Paleo, salty, Side Dish, Sweet Potatoes

Everyone brings a dish to share so lunch is always a beloved mish - mash of cultures — last year my mum's soya sauce duck with Chinese mushrooms fought for space on the overloaded table with my Auntie R's traditional roast turkey, my sister in law's clove - studded ham, our friend's Malaysian lamb curry, king prawns, oysters, my ex's German coleslaw and potato salad, noodles, smoked fish and quinoa salad brought by friends and my dad's famous lobster noodles.

Discovering dishes from different cultures, exploring new flavor combinations, and simply stuffing her face on some homemade comfort food are definitely past times that make her top five — along with scribbling about them in her notebook.

In addition to the delicious day - to - day options, guests can indulge in Wednesday Ethnic Inspirations tasting menus, which feature dishes that celebrate numerous cultures of cuisine, as well as afternoon tea times with desserts.

Filed Under: Breakfast, Brunch, Comfort Foods, Everyday Meals, Inspiration, Stories, Tea Culture, Uncategorized, Vegetarian Dishes Tagged With: Baking, bread, bread baking, brown bread, buttermilk, family history, heritage, immigration, International Women's Day, Irish, Irish brown bread, Irishness, March, pastries, rustic, Saint Patrick's Day, soda bread, whole wheat

Accompanying sauces have become more elaborate, fondue - like dishes from other cultures, such as Mongolian Fire Pots, are being served, unusual foods are being used and, of course, fondues are being fired - up with our favorite pungent pods.

The dinner courses include Maine peekytoe crab with pickled green tomato — jalapeño jam, almonds, and cultured cream and dry - aged cold spring ranch sirloin with spring succotash, as well as other innovative, Maine - sourced dishes.

As the Coast white paper explains, customers would rather a restaurant incorporate authentic ingredients and provide a faithful experience of a culture's food than dispense the mere simulation of a dish: «And those who can provide patrons with an authentic experience earn their trust and build a relationship with their consumers for the long - term.»

luckily his dad only knows how to make one indian dish so ive been able to get more creative with my cooking & venture out into food of different cultures.

Family meals are an important part of the day in any culture, but with today's busy lifestyles, we are more often grabbing things on the go and rushing to finish the meal with the minimum of dishes to wash up afterward.

Flavor, identity and culture are his culinary touchstones, and Chef Didier is known for his reinventions of classic dishes — with punk rock flair!

I really can't wait to share the discoveries with you and of course to cook some new dishes inspired by our ancestral cultures -LRB-!)

CC: With our menu, we like to take pieces of Latin culture and intertwine them into the menu, through cooking or preparing the dishes.

Can't wait to see your post and see how our different cultures unite with this dish!

A California native, Christina grew up in a culture that placed emphasis on dishes created around a diversity of fruits and vegetables with a wide variety of flavors.

U.S. Attorney Preet Bharara, in his typical machine - gun staccato delivery, pop culture references dished out with an arched eyebrow and declarations best suited for a James Ellroy noir, seemed more disappointed than angry at yesterday's news conference that he keeps having to police Albany.

But T. auensis is particularly hard to culture in Petri dishes and study with the normal tools of molecular biology.

In a culture dish microglia that were modified to make a lot of TREM2 gobbled more amyloid and removed more dying neurons, compared with microglia having less of the protein.

To grow these microorganism cultures in the lab, researchers house samples in petri dishes lined with a nourishing gel mixture derived from algae called agar growth media.

They studied H. pylori bacteria in a culture dish and compared the effect of only the antibiotic metronidazole against the bacteria versus the prodrug system with metronidazole and carbon monoxide combined.

When Stamets cultured the mold in his lab, a white circle of mycelium spread over the petri dish from the point of inoculation; it was soon covered with green spores.

Zheng, together with Leah Boyer, then a researcher in Gage's lab and now director of Salk's Stem Cell Core, generated diseased neurons by taking skin cells from patients with Leigh syndrome, reprogramming them into stem cells in culture and then coaxing them to develop into brain cells in a dish.

In cells grown on flat culture dishes, the expression of thousands of genes didn't match up with their normal patterns, explaining why the cells from those dishes had been unable to generate new hair follicles.

Rather than stick the isolated cells on a flat culture dish, they mixed the cells with liquid, then let the mixture hang in tiny droplets from a plastic lid, like condensation on the roof of a container.

«We culture typical skin cell of the epidermis, such as human keratinocytes, in our dishes to form an artificial epidermis with all of its natural layers,» explained Sibylle Thude, the biologist who led the investigation into the accreditation.

Studies of individual neurons in dishes of cultured neurons and in brain slices by co-first author Robert E. Stanley, a graduate student in the lab, revealed that neurons in the mutant mice had decreased numbers of dendritic spines, an important part of the synapses that neurons use to communicate with one another.

The investigators studied application of various doses of a common PBDE flame retardant on human adrenal cells in culture dishes and compared the effects with those of only the vehicle, the inactive substance used to deliver the chemical.

They then combined these in a dish with testicular cells from newborn mice in various culture conditions — a process that took hundreds of trials, says author Xiao - Yang Zhao, a stem cell biologist now at Southern Medical University in Guangzhou, China.

Duke scientists grew two sets of cellular «miniguts» on culture dishes and stimulated them with inflammatory factors.

They have spread lies about his methods, accused him of electrocuting monkeys when in fact he was working with cells in culture dishes, and limited his job opportunities.

The research builds on the team's previous work with a technique called three - dimensional culture, which involves incubating stem cells in a floating ball - shaped aggregate, unlike traditional cell culture in which cells grow in a flat layer on the surface of a culture dish.

Normally, CD4 cells will start dividing when they are treated with certain proteins in a culture dish.

COS - 7 cells, plated at 70 % confluence in 100 - mm tissue culture dishes, were transfected with vector alone or pcDNA3 - ACVR1 (c. 617G) or pcDNA3 - ACVR1 (c. 617A).

In initial work with cell cultures in Petri dishes, the researchers methodically ruled out the possibility that HIV / gp120 would be inducing the death of stem cells and determined instead that HIV / gp120 was acting by inhibiting stem cell proliferation.

Your culture dishes could be infected with Mycoplasma, tiny bacteria that thrive in cell culture environments.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Dissected brains were immersed in 200 μl of PBS and tethered with tungsten wire (0.125 mm diameter, A-M Systems, Inc.) to the bottom of a culture dish (35x10 mm, Falcon).

Confluent HFF cells were treated with irradiation of 55 Gy and plated onto culture dishes (1 × 105 per 2.89 cm2).

HepG2 cells in 100 mm cell culture dishes were transfected with pGL - MARE - Luc plasmid DNA along with plasmid carrying the human Bach1 gene or the empty vector pFLAG - CMV - 6c using Fugene6 (Promega).

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

Neuronal outgrowths in 24 - well tissue culture dishes were fixed, permeabilized and blocked as above prior to staining with anti-β-III tubulin, 1 ∶ 200 (Chemicon mab1637 followed by secondary labeling with AF488 goat anti-mouse IgG1 (A21121, Invitrogen).

SCR055, Millipore, Inc) in 24 - well culture dishes coated with poly - L - ornithine (20 µg / ml) and laminin (5 µg / ml).

Manipulating genes and then sorting through many cells to find the desired alterations wasn't possible with these cells because scientists couldn't grow them in the culture dishes where such maneuvers would take place.

Along with the common aversion to fruit and vegetables, there is often a reluctance to try new or different foods — for example, heavily herbed or spiced dishes or cuisines originating from an unfamiliar culture.