Cells (5 × 104) were seeded in 12.5
cm2 culture flask and incubated for 24 h to allow for cell attachment and recovery.
Briefly, the cortices of adult rat (SD) kidneys were collected, minced, and cultured
in culture flasks (NUNC, Roskilde, Denmark) using DMEM containing 10 % FBS.
Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2
tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).
Just about every single
cell culture flask / plate / dish, every tube or container, and all of the glassware has the words «A Corning Brand» written on it.
The enrichment method employed produced an almost homogeneous population of iPS - RPE cells at passage 2 in a 25 cm2
tissue culture flask (Fig. 1C) with no evidence of cell multi-layering.
In 1993, Dr. Wood began working with medical scientist Marie Stoner on a method to grow skin tissue directly on patients instead of in
a culture flask.
And, in fact, the bacteria, ranging in age from 100,000 to a stunning 8 million years old, came groaning back in
the culture flask.
Before passage,
the culture flasks were shaken to detach the more weakly adherent macrophages.