Sentences with phrase «culture flasks»

Cells (1 × 105) were seeded in 12.5 cm2 culture flasks and allowed for cell attachment and recovery.

Briefly, the cortices of adult rat (SD) kidneys were collected, minced, and cultured in culture flasks (NUNC, Roskilde, Denmark) using DMEM containing 10 % FBS.

The cells were routinely cultured in 25 - cm2 tissue culture flasks containing RPMI (ATCC, 30 - 2002) supplemented with 10 % fetal bovine serum (Biochrom) according to the supplier's instruction.

Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2 tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).

In 1993, Dr. Wood began working with medical scientist Marie Stoner on a method to grow skin tissue directly on patients instead of in a culture flask.

Cells (5 × 104) were seeded in 12.5 cm2 culture flask and incubated for 24 h to allow for cell attachment and recovery.

The enrichment method employed produced an almost homogeneous population of iPS - RPE cells at passage 2 in a 25 cm2 tissue culture flask (Fig. 1C) with no evidence of cell multi-layering.

Just about every single cell culture flask / plate / dish, every tube or container, and all of the glassware has the words «A Corning Brand» written on it.

Not exact matches

Like an eager first - grader playing show - and - tell, she's been known to pass around a flask of cultured cells so that visiting scientific reviewers can see the raw data and share her excitement.

The team established gut bacteria cultures in flasks and then «fed» them two different diets — either a predigested potato, high - starch diet or a predigested grass, high - fiber diet.

An accidental escape hinted at what those scents might be good for: «When returning to the lab after a weekend, I found that a flask with a smelly yeast culture was infested by fruit flies that had escaped from a neighboring genetics lab, whereas another flask that contained a mutant yeast strain in which the aroma gene was deleted did not contain any flies,» Verstrepen recalls.

Contrary to common practice, however, Ivins dumped material from 35 different anthrax cultures into a single flask, thus mixing a number of mutant strains.

For cell therapy, automation can be used to increase the scale of cell culture operations (e.g., bioreactors replacing flasks) and allow the use of closed systems that can protect cell products from contamination with adventitious agents from the environment or operators themselves.

Vector titers in hollow - fiber bioreactors have been demonstrated as comparable to those attained with flask or Nunc Cell Factory tray cultures: 1.7 × 108 viral genomes per milliliter (vg / mL) to 2.6 × 108 vg / mL (27).

Flask cultures were kept in a humidified incubator in a 5 % CO2 atmosphere at 37 ° C. Growth medium was replaced every 72 hours for two weeks.

Inverted microscopes are used to image lives cells in culture (flasks, dishes, or multi-well plates) using transmitted light, phase contrast, DIC, or fluorescence modes.

For induction of differentiation to mature endothelial cells, EPCs were plated at a high cell density (8 × 104 cells / cm2) on tissue culture treated flasks.

This primary culture was passaged through brief exposure to 0.05 % trypsin - EDTA (Invitrogen) and seeded onto gelatin coated 175 - cm flasks with fresh culture medium.

The show at Guild Hall includes images of a cryopreservation unit (where the dead are preserved by freezing), a glass flask containing live HIV cells, and a decomposing corpse that's part of the Forensic Anthropology Research Facility, known in popular culture as the Body Farm.

In microbiology, we have the most ideal tools of science available, allowing experimentation in small culture tubes or flasks, while physics is mostly math, and the experimentation is largely limited to the applied end where technology is developed.