Sentences with phrase «culture medium for»

In this test a sterile toothbrush is used to brush the cat's hair over a wide area and then the bristles are pressed on the surface of a special culture medium for dermatopyhtes (the ringworm organism).

Culture media for tumor cells were supplemented with 10 % FBS, whereas culture medium for HUVEC was supplemented with 20 % FBS, with the exception of the experiment under low proliferation rate conditions (2 % FBS).

When glucose is added to the culture medium for cows and sheep embryos, a greater number of males survive.

The objective of the Islamic slaughter is to ensure maximal blood drainage from the animal as Muslim belief blood is a good culture medium for microorganisms to flourish.

Not exact matches

Without further ado, we present you our medium - sized company category for our Top Company Cultures list.

Entrepreneur teamed up with CultureIQ to rank high - performance cultures existing at medium - sized companies for our Top Company Culturcultures existing at medium - sized companies for our Top Company CulturesCultures list.

On June 30, star investor Chris Sacca wrote on Medium that he took some personal responsibility for «the unrelenting, day - to - day culture of dismissiveness that creates a continually bleak environment for women and other underrepresented groups» in Silicon Valley (shortly before allegations of his own sexual misconduct emerged in the same piece that broke the story about McClure).

Written for EO by Marina Byezhanova, co-founder and director at Pronexia Inc., headhunters for small - to medium - sized businesses and company culture consultants.

«We are disappointed to see that Uber has selected a team of insiders to investigate its destructive culture and make recommendations for change,» they wrote in an Medium post published on Thursday, referring to the fact that Holder's firm has close ties to Uber and he previously advocated against fingerprint - based background checks for Uber drivers.

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

In 2017 alone, Entrepreneur Magazine ranked us on the list of Best Company Cultures, Austin Business Journal voted us 8th Best Place to Work (Medium Category), and we won a Stevie Award for Great Employers.

Kathryn contributes essays, culture coverage, and reporting for national publications like Medium, PASTE, and Brit + Co..

Not only must one view the individual patient as an operating biological organism, one must also seek to understand both the environing medium for that person, which includes all other persons with whom functional activity occurs, and the specific culture that to a large extent shapes the perceptual patterns by which that individual experiences the world.

For years, the prestige TV movement has helped the medium of serialized storytelling become pop culture's most important channel for engaging big ideas and sparking social dialogue the way novelsFor years, the prestige TV movement has helped the medium of serialized storytelling become pop culture's most important channel for engaging big ideas and sparking social dialogue the way novelsfor engaging big ideas and sparking social dialogue the way novels...

The school stands as the only mass medium capable of putting forward the case for what is not happening in the culture.

Ghee rendered from cultured butter is Chef Tory Miller's frying medium of choice for the fingerling potato chips that go with his beef tartare: «The chips get super crispy, with that buttery funkiness,» says Miller, the chef at Graze, an eclectic gastropub in Madison, Wisconsin.

The fertilised egg (zygote) cultured for 2 - 6 days in a growth medium and is then transferred to the mother's uterus with the intention of establishing a successful pregnancy.

Samples collected from surfaces and from the air are cultured on plates containing a growth medium, one specific for bacteria and another for fungi.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

Human chorionic gonadotropin was detected in the medium surrounding two embryos cultured for more than 7 days after fertilization.

The resulting organoids could either be banked for future use or immediately fragmented and seeded into the «epithelial» channels of multiple Organ Chips where they were further matured by flowing specialized culture medium and applying mechanical stimulation to the channels to mimic physiological peristalsis - like motions.

One reason for the persistence of this pyramid of production is that for the past half - century, much of the world's media culture has been defined by a single medium — television — and television is defined by downloading.

Isolated islets from 3 - wk - old Tg - hIAPP, which do not exhibit IAPP aggregates at that age (Janson et al., 1996), were cultured in the presence of 1 % or 0.1 % islets extracts from old Tg or WT mice for 7 d under standard conditions, including a glucose concentration of 11 mM in the medium to reduce glucotoxicity and spontaneous aggregation of the protein (Zraika et al., 2007).

These 2D cultures also abolish the need for intraluminal microinjection of single organoids and facilitate studies of host - microbe or virus interactions or the action of microbial enterotoxins because the luminal surface is fully exposed to the mucosal medium (22,23).

StemFit Basic02 is a xeno - free, defined medium for human pluripotent stem cell (hiPSC) culture that offers an effective solution for regenerative medicine research.

Then, cells were either stimulated with methionine - choline deficient media (MCD - media; WME without choline, methionine, or glutamine and with 0.1 % BSA) for 24 hr or cultured in control medium (WME with 0.1 % BSA).

Before determining glucose uptake, all cell cultures were exposed to basal medium for 1 h. Cultures used for acute insulin stimulation were exposed for 1 h to high insulin as described for GScultures were exposed to basal medium for 1 h. Cultures used for acute insulin stimulation were exposed for 1 h to high insulin as described for GSCultures used for acute insulin stimulation were exposed for 1 h to high insulin as described for GS assays.

After 2 — 3 weeks, propagated cells were dissociated with 0.25 % trypsin, resuspended with culture medium, and cultured for a further 2 — 12 weeks.

Expansion of CD34 + cells normalized relative to the values obtained in SFEM medium (dark gray bar) after culturing purified CD34 + cord blood cells for 7 days in StemSpan ™ serum -[SFEM, SFEM II (gold bar)-RSB- and animal component - free ACF (orange bar) media, and six media from other suppliers (light gray bars).

One - half of the culture medium was replaced with a fresh medium every 3 — 4 days for the 16 DIV.

StemSpan ™ serum - free medium undergoes rigorous QC testing to ensure consistent conditions for the culture of HSPCs.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Two days later, 2i and N2B27 plus LIF replaced the HFF medium and the culture lasted for another three days.

After 6 — 7 days of suspension culture, the neurospheres were replated onto matrigel - coated 60 mm tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

For induction and culture of mouse iPS cells, HFF cells were passaged using trypsin and cultured in medium containing DMEM, 10 % FBS (Hyclone), 1 % NEAA (Invitrogen), 2 mM L - glutamine (Invitrogen), 100 U / ml penicillin and 100 µg / ml streptomycin.

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

For example, Masato Kanemaki's lab has developed a CRISPR / Cas - based system for tagging endogenous proteins with an auxin - inducible degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture mediFor example, Masato Kanemaki's lab has developed a CRISPR / Cas - based system for tagging endogenous proteins with an auxin - inducible degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture medifor tagging endogenous proteins with an auxin - inducible degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture medium.

For monolayer differentiation, confluent iPS cells were cultured in the HFF culture medium without any growth factors.

Each of these tissues was also processed fresh for culture in standard (BSK - H) medium.

A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days.

Flask cultures were kept in a humidified incubator in a 5 % CO2 atmosphere at 37 ° C. Growth medium was replaced every 72 hours for two weeks.

After three weeks, all medium from the six - well cultures was removed, followed by a single media wash and replacement with fresh medium for 24 hours.

After 3 days of RA treatment, the EBs were plated on the matrigel - coated culture dishes in the N2B27 medium for several days.

Primary neurospheres were then collected and replated onto matrigel - coated tissue culture dishes for adherent culture in the same medium for another 2 — 3 days.

Cells were plated in six - well plates at 2.5 × 105 (A427) or 4 × 105 (H2122) cells / well in RPMI 1640 medium containing 10 % FBS and cultured for 24 h. Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemicals).

In the case of EB formation using mouse testicular or ovarian cell - conditioned medium (see below), the EBs were cultured in ES medium for 24 hr, and then in a conditioned medium.

It has been demonstrated that the composition of the medium used for embryo culture has a profound effect on the methylation pattern in the resultant two - cell embryos [10], indicating that, in addition to imprinted genes, other epigenetic alterations may intensely modify gene expression.

To derive NSCs as previously described [11], hESC colonies were harvested using a scraper and cultured in suspension as EBs for 8 days in ESC medium minus FGF2.

For postmitotic neurons, we chose to use an established neuronal differentiation culture system in which NSCs were induced to differentiate into dopaminergic neurons by medium conditioned on stromal cells for 4 weeFor postmitotic neurons, we chose to use an established neuronal differentiation culture system in which NSCs were induced to differentiate into dopaminergic neurons by medium conditioned on stromal cells for 4 weefor 4 weeks.

nPTLS patients then had a lumbar puncture and CSF was evaluated for cell count, total protein, glucose, total gammaglobulin, oligoclonal bands and evidence of B. burgdorferi (ELISA, Bb DNA by PCR, and culture using BSKII medium).