Sentences with phrase «culture medium from»

None of these toxic effects were observed when the same cells were treated with culture medium from non-senescent astrocytes that had not been subjected to paraquat.7

Not exact matches

What I'd like us all to take from «wm» is a conviction that conservative pop culture studies have to attend to the impact of the medium as well as of the message, or better said, since McLuhan can't be fully right, that we must grapple with the popularity as well as the culture of pop culture.

With some entailment of that danger always implicit in superlatives, one may raise the question whether any other single contribution from whatever source since human culture emerged from the stone ages has had the far - reaching effect upon history that Israel in this regard has exerted both through the mediums of Christianity and Islam and directly through the world of Jewish thinkers themselves.

The objective of the Islamic slaughter is to ensure maximal blood drainage from the animal as Muslim belief blood is a good culture medium for microorganisms to flourish.

Ghee rendered from cultured butter is Chef Tory Miller's frying medium of choice for the fingerling potato chips that go with his beef tartare: «The chips get super crispy, with that buttery funkiness,» says Miller, the chef at Graze, an eclectic gastropub in Madison, Wisconsin.

Starter cultures often include whey, kefir, yogurt, or left over fermentation medium from the previous batch.

Kombucha, which many of you are likely familiar with, differs from water kefir because kombucha is specifically cultured in a tea and sugar medium, and water kefir can be cultured using just water and sugar.

Samples collected from surfaces and from the air are cultured on plates containing a growth medium, one specific for bacteria and another for fungi.

When the Stanford team measured pyrophosphate levels in cultured cells derived from ank and normal mice, they found that the chemical accumulated in cells from the ank mice but decreased in the culture medium.

Diluted and filtered liquid medium drawn from the fibroblast cell culture also killed breast and lung cancer cells.

Draths and Frost were able to complete the final stage, in which muconic acid is converted to adipic acid by hydrogen gas and a platinum catalyst, without having to separate the muconic acid from the crude culture medium.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

The bacteria would need either to synthesize the new genetic letters themselves or to import them from the surrounding culture medium.

The kit adopts a novel purification method, using magnetic beads and phosphatidylserine - binding protein to isolate exosomes and other EVs from cell - culture medium and body fluids by a normal microcentrifuge.

The growth potential of excised embryos cultured on 1/2 MS medium declined by 31 % after 1 month of dry storage or after 24 h of dry storage at − 18 °C (Table 3), but growth was similar to that of embryos from freshly collected seeds (c. 80 %) when embryos were cultured with growth hormones.

When NGF is withdrawn from the culture medium, the cells retract their fibers, lose their other neuronal properties and resume the uncontrolled proliferation characteristic of neoplastic cells.

Isolated islets from 3 - wk - old Tg - hIAPP, which do not exhibit IAPP aggregates at that age (Janson et al., 1996), were cultured in the presence of 1 % or 0.1 % islets extracts from old Tg or WT mice for 7 d under standard conditions, including a glucose concentration of 11 mM in the medium to reduce glucotoxicity and spontaneous aggregation of the protein (Zraika et al., 2007).

Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2 tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed).

Human brain vascular pericytes (BP) were purchased from ScienCell (# 1200) and cultured in pericyte medium (ScienCell, # 1201) supplemented with 2 % FCS, 1 % of the corresponding pericyte growth supplement and 1 % penicillin / streptomycin.

HUVEC were purchased from PromoCell (C - 12203) and cultured in Endopan 3 medium (PAN Biotech, #P04 -0010 k)-RRB- supplemented with 3 % FCS, the corresponding supplement mix and 1 % penicillin / streptomycin.

Dermal fibroblasts were purchased from PromoCell (C - 12300) and cultured in Dulbecco's modified Eagle medium (DMEM)(Gibco) supplemented with 10 % FCS and % penicillin / streptomycin.

Expansion of CD34 + cells normalized relative to the values obtained in SFEM medium (dark gray bar) after culturing purified CD34 + cord blood cells for 7 days in StemSpan ™ serum -[SFEM, SFEM II (gold bar)-RSB- and animal component - free ACF (orange bar) media, and six media from other suppliers (light gray bars).

Vero E6 tissue cultures [obtained from The American Type Culture Collection (ATCC), CRL: 1586] were grown in Dulbecco's modified minimum essential medium (DMEM) supplemented with penicillin (100 units / ml), streptomycin (100 µg / ml), 0.2 % sodium bicarbonate and 10 % fetal bovine serum (FBS).

Bone marrow cells cultured with conditioned medium derived from ABL1 / ABL2 knockdown 1833 and SKBR3 breast cancer cells had decreased numbers of TRAP + cells compared to the control groups (Fig. 5, B and C, and fig.

Culturing wild - type brains with the fat bodies from fed gbp1, gbp2 ex67 larvae showed high levels of ILP2 and ILP5 accumulation that were indistinguishable from those from brains cultured in medium alone or with starved fat bodies.

By activating a gene called Ras in cells bathed in a very specific culture medium, scientists were able to cause embryonic stem cells — which originate from the inner cell mass of the blastocyst — to become more like the trophoblastic stem cells that give rise to the placenta from the outer portion of the blastocyst.

The human breast cancer cell line SKBR3 was purchased from the Duke University Cell Culture Facility and was maintained in McCoy's 5A medium (Life Technologies) supplemented with 10 % FBS (Life Technologies) and antibiotics.

On the other hand, the fat bodies from starved wild - type larvae showed high levels of ILP2 and ILP5 accumulation, comparable to levels of accumulation in brains cultured in medium alone without fat body.

We standardized the densities of ILPs by fixing the values from the «no fat body» treatment (culturing with a plain medium) to 1.

Peptides were added to the culture medium, and fluorescence changes from GCaMP6s were measured by live imaging.

After three weeks, all medium from the six - well cultures was removed, followed by a single media wash and replacement with fresh medium for 24 hours.

Most notably, however, parthenogenic blastocysts were only produced from embryos cultured with supplementation and not with Sage or IVM - medium alone (Table 2 and Figure S2).

These inserts are added to wells containing complete culture medium with 10 % FBS as chemoattractant in 24 - well fluorescence opaque plates allowing only monitoring of fluorescence from the bottom.

Human astrocytes were purchased from Sciencell Research Laboratories (isolated from human cerebral cortex, Cat # 1800, Carlsbad, CA) and were cultured in human astrocyte medium (Sciencell, Cat # 1801) on poly - L - lysine coated tissue culture dishes.

Add the starter culture: In a medium bowl, stir together the active yogurt and ¹ ⁄ ₂ cup of the warm milk from the pressure cooker basin.

This rich culture medium (PRE-BIOTICS) is then encapsulated along with the Probiotics that have grown together over the three - year period and the resulting nutrients from this fermentation process (POST-BIOTICS), thus delivering viable probiotics and encouraging your body's innate ability to grow its own friendly bacteria.

They disregard popular culture and refuse to acknowledge the literary power of any writing that deviate from the common novel, play, or poem medium,» says Sellars, noting his plans to embrace pop culture and bring comics, graphic novels, manga, anime, and video games into the K — 12 classroom.

Learn how to rephrase the performance objectives of college and career ready standards for the visual, performing, and media arts into good questions that will engage students to create, perform, respond to, and connect works presented in various formats and mediums, by various artists, in different contexts, and from different cultures.

Because Tezuka was so influential in the manga and anime world, it's also its own history on these two mediums from about the 1940s to the 1980s, showing the mechanics of how they were done at the time, the culture around them, and the ways they developed and excelled.

Your veterinarian will pluck hairs from your cat and put them in dermatophyte test medium (DTM) culture (the most accurate test) to see if the fungus will grow.

Hairs from affected areas are plucked with a sterile hemostat and placed on a special fungal culture medium.

Your veterinarian will pluck hairs from your pet's fur to perform a fungal culture using a dermatophyte test medium (DTM) plate or jar and conduct a Wood's lamp examination, which involves shining a specially coated fluorescent light on your pet's fur.

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)

A Profound Waste of Time is a new magazine from the UK, focusing on — says its successful Kickstarter campaign blurb — «a bold new video game culture magazine, a lovingly produced home to great writing on the medium and its accompanying narratives».

Since the 1960s — fuelled by the civil rights movement, reactions to the Vietnam war and second - wave feminism — contemporary art has become an intrinsically politicised, critical medium through which everything, from culture to capitalism and the medium itself could be questioned and deconstructed.

Drawn from the Christopher E. Olofson Collection, Revealing Pictures displayed contemporary photographic works engaging with the medium's ability to articulate complex issues of national identity, culture and resistance, both personal and beyond.

(251.1 x 251.1 cm) Medium: Oil on canvas Credit Line: Purchased with funds from the North Carolina State Art Society (Robert F. Phifer Bequest), the North Carolina Museum of Art Guild, and various donors, by exchange Object Number: 96.2 Culture: German Signed: Verso, right center: Richter 1985 Inscriptions: Verso, near top left: 577 - 2 Classification: Paintings Department: Modern

As contemporary artists feed an image - hungry culture, making and disseminating pictures with unprecedented speed on every possible platform, photo curators are tasked with picking and choosing from a glut of wildly multifarious works that can claim to be associated with the medium.

Working across mediums, they looked at cross-collaborations between fashion, music, popular culture, and art: from Jay - Z performing with artist Marina Abramovic at MOMA, to David Bowie using cut - up techniques to create song lyrics.

At no time was this more evident than in the later»60s, when, fed up with reductive critiques that related his sculptures to car crashes and thence to the violence supposedly endemic to contemporary American culture, Chamberlain took a seven - year sabbatical from his signature medium.