Sentences with phrase «culture plate into»

Not exact matches

Just as the clash of these geological plates causes earthquakes, so we can expect massive cultural earthquakes as the great cultures are forced into closer contact.

This demonstrates that even though the anterior neural plate is essential for proper eye morphogenesis, cultured pluripotent cells were not directed to a retinal fate — even when grafted directly into the eye field.

Delaney et al. [4] who have shown that cord blood - derived CD34 + cells cultured in StemSpan ™ medium with cytokines, on plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.

Assays for virus - specific neutralizing antibodies were performed on serial 2-fold diluted samples of each serum using 2 % FBS - DMEM as the diluent in 96 - well tissue culture plates (Falcon 3072); the final volume of the serially diluted samples in each well was 60 µl after addition of 120 TCID50 of SARS - CoV in 60 µl into each well.

For intracardiac injections, cells were harvested from subconfluent culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28) in PBS; 0.1 ml of the suspended cells was injected into the left cardiac ventricle using 30 - gauge needles.

We induced differentiation of hPSCs into the mesodermal lineage by culturing on collagen - coated plates with a glycogen synthase kinase 3β inhibitor.

The cells were then seeded into 12 - well culture plates (1 ml / well; Falcon, Franklin Lakes, NJ).

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

2014 Scott, Ronnie, David Shrigley behind the lines, The Saturday Paper, 29 November Ewing, Sarah, David Shrigley on his childhood, Adam and the Ants and Glasgow School of Art, The Independent, 31 October Scott, Fiona Sinclair, David Shrigley Revamps London's Sketch Restaurant, Showcasing Largest Body of Work Ever, Huffington Post Arts & Culture, 13 June Dickens, Zoe, In Conversation with David Shrigley, Harper's Bazaar, 11 June Brown, Griselda Murray, David Shrigley interview, Financial Times, 10 June Compton, Nick, Artist David Shrigley turns Sketch's Gallery restaurant into a work of art, Wallpaper, 10 June Curtis, Jake, Art on a Plate, 8 June Sherwin, Skye, Food for Thought, W Magazine, 6 June Jury, Louise, David Shrigley dines out on his designs for Sketch, London Evening Standard, 6 June Lewis, Tim, Q&A New Review, The Observer, 1 June Lewis, Tim, David Shrigley: «It's difficult in the world of fine art to have a comic voice», The Guardian, 31 May Edwards, Rhiannon, Brit Art conquers America, The Telegraph, 11 April Brown, Mark, High art: Thumbs up in Trafalgar Square, The Guardian, 8 February Singh, Anita, Fourth Plinth statues unveiled: a giant thumb and a horse skeleton, The Telegraph, 7 February