Sentences with phrase «culture plates»

F1 virgin females were collected, transferred to individual cells of plastic 24 - well culture plates (various manufacturers) and given a single fly host to lay eggs.

If the cells grew on culture plates, the team inferred that the human gene could fill in for its yeast equivalent.

The researchers removed the organ of Corti, which is housed within a seashell - shaped cavity called the cochlea and contains sensory hair cells, from newborn mice and kept the cells alive in culture plates.

Functional and transcriptional comparison of iPSC - Hep 3D cultures plated as single cells or clumps.

Two petri dish culture plates show the interaction of bacteria and antibiotics (on disks).

For intracardiac injections, cells were harvested from subconfluent culture plates, washed with phosphate - buffered saline (PBS), and resuspended at 106 / ml (1833) or 5 × 106 / ml (SCP28) in PBS; 0.1 ml of the suspended cells was injected into the left cardiac ventricle using 30 - gauge needles.

Using a multichannel pipette, transfer 5µL of cells from each well of the 96 well cell culture plate into a PCR plate containing 25 µL / well of «direct lysis buffer» (from step 3).

Briefly, iPS - RPE were cultured on gelatin - coated culture plate inserts.

EPCs obtained after isolation generally adhere to culture plates within 6 to 8 hours of isolation.

A positive culture result obtained from a tracheobronchial wash does not necessarily imply the presence of a clinically significant airway infection and should not lead to antibiotic therapy, unless there was a pure bacterial culture on a primary culture plate.

«Before, you could in principle test chemical compounds of interest against hundreds of cell lines, but it would require a lab member going to a freezer, taking out hundreds of vials — one for each cell line — and setting up hundreds of tissue culture plates.

This protocol video demonstrates how to surface - coat cell culture plates and transwell inserts with Cultrex ® Basement Membrane Extract, also called Cultrex ® BME.

Co-senior author Rohit Jindal, Ph.D., of MGH - CEM, adds, «The microfabrication methods applied in this study are readily amenable to designing a device in which multiple two - chamber wells could be operated at the same time, dramatically increasing processing speed, that would be no larger than a standard, 96 - well culture plate.»

The researchers used a type of fiber specifically indicated for absorbing the aromatic compounds from the culture plates.

This is a culture plate of Lactococcus, milk fermentation tubes and mini-cheeses made in Dr Mark Turner's lab.

I wouldn?t miss the test tubes and tissue culture plates, but a future without these miniature miracles would definitely look bleak.

After collecting biopsies from banked human donor eyes, the team expanded the numbers of cells in a culture plate using human serum to nourish them.

The research highlights the importance of studying the effects of drugs not only on the tumor cells in a culture plate, but also on the interaction of the tumor cells with their own microenvironment.

Modeling early heart development is difficult to achieve in a petri dish and tissue culture plates, the study authors said.

Like a conventional tissue culture plate, DA - HCS plates can be easily coated with DNA, poly - lysine, Collagen, fibronectin, silane - compatible substrates, or any other cell culture coatings.

Comparison of Human alveolar osteoblasts cultured on polymer - ceramic composite scaffolds and tissue culture plates.

For cell proliferation assay, 5 × 104 cells were seeded in triplicate wells per cell line per time point in 12 - well tissue culture plates.

The incubation gases are piped directly onto the culture plate through a perfusion lid.

NHLF cells were plated in complete medium at 4000 cells / well in 96 - well culture plates (BD Falcon) one day prior to silencing.

Briefly, NHLF cells were grown in 96 - well CoStar tissue culture plates (4,000 cells per well) and either subjected to siRNA gene silencing or directly treated with compounds for 51hours in 100 µL complete medium per well.

Briefly, cells grown in 96 - well tissue culture plates were exposed to compound for 4 hours after which cells were lysed with the provided Total Glutathione Reagent and luminescence was determined using a SpectraMax 384 plate reader (Molecular Devices).

Primary cultures were plated on poly - d - lysine (Sigma) coated tissue culture plates.

Since the surface properties of ePTFE are much different than tissue culture plates, we carried out adhesion assays on 1 cm2 ePTFE rafts.

In vitro assays are performed on tissues or cells maintained outside of the body, usually in the form of a cell culture plate or test tube.

Then, 100 μL single - cell suspension was dispensed into each well in a 96 - well culture plate.

HEK293T cells were seeded at 2 × 107 cells in 15 - cm tissue culture plates and cells were treated with 20 mM LiCl for 7 hr.

The current method is time - consuming, requires the time of scientists and lab staff, and is limited to organisms that grow on a culture plate.

Biomedical waste, such as culture plates, tubes, contaminated sponges, swabs, biologicals, blood - borne pathogens, needles, syringes, and blades, must be disposed of according to federal, state, local, and provincial regulations.

After starting to attempt to lift itself on day 6, SB - 1 started to slowly crawl on the agar surface of the culture well on day 9, and started to eat the algal food provided the culture plate on day 13.