Sentences with phrase «culture supernatant»

For initial optimization of virus testing of tissue culture supernatants and allantoic fluids, RNA was isolated manually using the high pure RNA isolation kit (Roche Diagnostics, Almere, The Netherlands) according to instructions from the manufacturer.

(B) Using ELISA, IFN - γ was measured from culture supernatants of (A) after 24 or 72 h of stimulation.

However, despite multiple serial passages, we were unable to propagate the infected cell culture supernatant in either BSC - 1 or PMK cells.

Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα in culture supernatant of ATRA - treated NB4 cells.

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

His - tagged recombinant merozoite proteins were purified from spent tissue culture supernatants by using a custom - built, piston - driven, sample - loading apparatus as described previously [33].

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

Immunoglobulin - containing HB1.F3.H2IgG NSC culture supernatant was subjected to purification by protein A affinity chromatography.

Combined culture supernatant and deoxycholate reduced calcium uptake by 39 % (p less than 0.001) suggesting a potentiation of supernatant activity by deoxycholate.

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

Allantoic fluid and the cell culture supernatants from the first five passages were tested for hemagglutinating activity with a 1 % suspension of chicken erythrocytes to exclude avian hemagglutinating viruses (e.g., influenza virus and EDS - 76 virus).

T cell lines were maintained by biweekly stimulations in complete DMEM supplemented with 10 % prescreened FCS and 5 % conditioned medium that was prepared from culture supernatant of rat splenocytes stimulated with Con A for 48 h.

(E and F) Using ELISA, IL - 2 and IFN - γ production were measured from culture supernatants of (D) after 72 h of stimulation.

Our lab uses this kit routinely to to quantify p24 levels in culture supernatant.

Extracellular vesicles (EVs) such as exosomes (70 nm — 160 nm in diameter) and microvesicles (100 nm — 1,000 nm diameter) can be harvested from cell - culture supernatants and from all bodily fluids.

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells

Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet.

Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will...

can confirm that ab16669 CD3 antibody [SP7] is sold as tissue culture supernatant.

I can confirm that the Anti-CD3 antibody [SP7](ab16669) antibody isprovided as tissue culture supernatant.

Briefly, 100 ml of culture supernatants were mixed with AVL - Carrier RNA buffer.

The enveloped viral particles were also observed in negatively stained samples prepared from the concentrated cell culture supernatants and allantoic fluids (Fig. 4F).

For cell culture supernatant, levels of IFN - γ and IL - 17A were measured by ELISA, according to the manufacturer's instructions (R&D Systems).

Insets are magnified virions (E); Negative staining of the purified BYDV from the cell culture supernatants (arrow indicates the typical enveloped flavivirus)(F).

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.

Twelve hours after transfection, the cells were incubated for another 24 h in the culture supernatants of NB4 cells treated with or without ATRA.

The IFNα level in the culture supernatant of IRF - 1 — transfected U3A cells was 3-fold elevated when compared with control cells (Fig. 3C).

The cell culture supernatant was collected and cytokine secretion was determined using the Mouse IFN - gamma Quantikine ® ELISA Kit (Catalog # MIF00), the Mouse IL - 4 Quantikine ® ELISA Kit (Catalog # M4000B), and the Mouse IL - 17 Quantikine ® ELISA Kit (Catalog # M1700).

Thirty - six hours after transfection, the culture supernatants were collected and used for IFNα titration.

The culture supernatants corresponding to every time point were used for IFNα concentration quantification.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

The harvested tissue culture supernatants (~ 200 mL for each protein) were supplemented with imidazole (10 mM) before loading each well at 1 mL / min.

A high - throughput, custom - made purification platform was successfully used to purify recombinant proteins from large volumes of tissue culture supernatants to permit systematic comparative studies of purified antigens [33].