Sentences with phrase «culture supernatants of»

(E and F) Using ELISA, IL - 2 and IFN - γ production were measured from culture supernatants of (D) after 72 h of stimulation.

Mcc was purified from the culture supernatants of E. coli VCS257 cells harboring pJEM15 plasmid, as described earlier (Bieler et al., 2005).

Because ATRA could cause IFNα synthesis and secretion in NB4 cells by up - regulating IRF - 1 (23), we thus quantitated the IFNα in culture supernatant of ATRA - treated NB4 cells.

Not exact matches

Hybridoma supernatants were screened for anti-rGRF activity by use of a pituitary culture assay system that can detect growth hormone - releasing factor in the femtomole range.

After achieving 80 — 90 % confluency, cell culture media were changed to maintenance media with 2 % FBS and were inoculated with 200 µL of clinical sample or 100 µL of passaged viral supernatant.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

Both technologies allow the simultaneous detection of multiple factors in serum, plasma and tissue culture supernatants.

Cultured pericytes abundantly expressed calpain1 (Fig. 2d) and showed a significant induction of calpain1 activity after stimulation with supernatants of Ang2 overexpressing HUVEC (Fig. 2e).

Angiopoietin - 2 protein levels of cell culture supernatants were measured by ELISA (R&D, #DANG20) according to the manufacturer's instructions.

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

Briefly, 100 ml of culture supernatants were mixed with AVL - Carrier RNA buffer.

Assessment of hASC - CM composition found high expression of various human growth factors (IL ‐ 6, 8, 12, eotaxin, IP10, MCP ‐ 1, VEGF, and TIMP ‐ 1) in the supernatant following the co-culture of hASCs with islet cells, while IP10, eotaxin, VEGF, and TIMP ‐ 1 became increased with time during islet co ‐ culture, suggesting the presence of paracrine cross ‐ talk between islets and hASCs.

To characterize the isolate that replicated in HeLa cells, a 166 - bp RNA sequence containing the variable region C of the envelope (Env) surface protein was PCR - amplified from infected HeLa cell tissue culture supernatants.

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

0.1 mL of supernatant was added to BHK - 21 and African green monkey kidney (Vero) cell cultures (ATCC, Manassas, VA) and monitored as above.

In addition, we showed that, although the supernatants from each of the HCMV - infected cultures contained infectious virus, the viral copy number and the quantity and timing of virus production varied among the various organ - specific MSC.

These changes were accompanied by severe EC damage, decreased E-cadherin RNA level, elevated IFN - gamma in splenocyte culture supernatant, and production of significant IgM antibody against intestinal microbiota.

The supernatant, the medium in which the culture is grown, contains a multitude of beneficial byproducts of the growth process, including vitamins, enzymes, antioxidants, and immune stimulators.

Combined culture supernatant and deoxycholate reduced calcium uptake by 39 % (p less than 0.001) suggesting a potentiation of supernatant activity by deoxycholate.