Sentences with phrase «cultured cell lines»

Hence, its members favor primary cell lines and try to avoid experiments with cultured cell lines, they favor three - dimensional cell cultures over two - dimensional cell monolayers that are cultivated on hard and flat surfaces and they try to maintain the three - dimensional context of plants, cell clusters, tissues sections and small animal embryos.

An analysis of all gene transcription in the cultured cell lines turned up a large gene complex in which gene expression differed conspicuously in cells from patients compared to controls.

They confirmed low levels of miR - 184 expression in human glioma tissue samples and cultured cell lines as well as an increase in the expression of SND1 compared to normal brain tissue.

Potti and his colleagues began by testing chemotherapy drugs on cultured cell lines from human tumors, such as from the lung, breast, or ovary.

The scientists also generated a panel of (reconstructed) ancestral and existing TRIM5 genes (19 total), expressed them in cultured cell lines, and exposed the cells to 16 different retroviruses (lentiviruses and others) to see which TRIM5 versions conferred resistance to which viruses.

This is the route our project is pursuing, but it may take several years to establish a cultured cell line; bird primordial germ cells are quite finicky about living outside of a bird's body.

Not exact matches

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

«When someone has a problem — whether they need to find a particular cell line or need to understand the formulation of a particular type of culture medium — they're coming to you and they're in a crisis.

CCG is also developing cell lines that are cultured to represent tumor behavior in vivo, and computational methods that will help researchers understand tumor behavior and apply the resulting data to diagnoses, treatments, and cures.

What's more, those increases lined up with the cell culture data.

If a breast cancer line had silently contaminated Klonisch's cell culture early on, it would have been subject to the usual technique for immortalizing a normal cell (which involves applying enzymes, antibiotics and antibiotic - resistant genes).

Until recently, Ain was renowned for a highly prized repository of 18 immortal cancer cell lines, which he developed by harvesting tissue from his patients» tumors after removal, carefully culturing them to everlasting life in vials.

Through these effects, the PERY peptide reduced the proliferation of several (but not all) cancer cell lines in culture and inhibited the growth of a human breast cancer xenograft in mice.

With the help of my colleagues I started growing retroviral vector - containing plasmids in bacterial cultures, isolating the plasmid DNA and transfecting special producer cell lines with these plasmids.

Part of the trouble is the ingredients: Subtle variations in tissue - culture chemicals and Matrigel, or in different stem cell lines and how they are grown first in 2 - D culture, can have a big impact on how the organoids turn out, Novitch says.

«We compared the ability of RSV and parainfluenza virus (PIV3)-- another common virus in children that causes much less severe airway disease — to infect and cause inflammatory responses in a cell culture model of human epithelial cells, which compose the lining of the lung airway.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

By functionally linking the signal transduction of melanopsin to the control circuit of the nuclear factor of activated T cells, we have designed a synthetic signaling cascade enabling light - inducible transgene expression in different cell lines grown in culture or bioreactors or implanted into mice.

However, Eggan and McCarroll emphasized that now that this phenomenon has been found, inexpensive gene - sequencing tests will allow researchers to identify and remove from the production line cell cultures with concerning mutations that might prove dangerous after transplantation.

She added bacterial cells to a culture of a widely used mammalian cell line derived from the ovaries of Chinese hamsters.

A cell line consists of cultured cells that often originate from a tumor.

Now that they have shown how this defense mechanism works in cultured cells, they hope to study the importance of LINE - 1 jumping and the defenses against it in specific diseases.

Lacking the expensive machinery needed to validate cell lines on their own, most researchers either skip validation or ship their cultures to specialized labs which can delay important findings and treatments.

«In future, the decoded hamster genome will greatly advance the use of cell lines to produce pharmaceuticals», says Noll, who runs the Cell Culture Technology research group at the Faculty of Technology and participated in the research projcell lines to produce pharmaceuticals», says Noll, who runs the Cell Culture Technology research group at the Faculty of Technology and participated in the research projCell Culture Technology research group at the Faculty of Technology and participated in the research project.

In contrast to other cultured cells, such tumor cells can divide indefinitely and a cell line can therefore be cultured for many years.

Cells from her malignancy were cultured and used to start a cell line, called HeLa, which lives on to this day in research labs around the world.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

All human cell lines growing in tissue culture dishes, and about 95 % of cells in a human being, split evenly, resulting in daughter cells of equal size.

«Using this cell line allows us to separate 2 - cell - like cells from the ES cells in the culture by collecting the green cells which have entered the 2 - cell like state.

Following up on clues — including the fact that PMS is 56 percent heritable — the NIH researchers studied the genetic control of gene expression in cultured white blood cell lines from women with PMDD and controls.

The group reported growing multiple parthenogenetic embryonic stem cell lines by incubating eggs in a warm, low - oxygen culture medium.

We purchased MDA - MB - 231 (MB231) and MCF7 breast cancer cell lines from American Type Culture Collection.

We next attempted to culture TMAdV in an A549 (human lung adenocarcinoma) cell line, a BSC - 1 (African green monkey kidney epithelial) cell line, and PMK (primary rhesus monkey kidney) cells (Fig. 5).

Individual cultures of 3T3 (a murine fibroblast line) and endothelioma cells subjected to identical treatment were used for negative and positive controls, respectively.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

The immortalized endothelial cell lines established from H - 2Kb - tsA58 mice provide, for the first time, a cell culture system to examine factors regulating angiogenesis and tumor cell arrest in different organ systems.

AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting of the neuron - specific class III b - tubulin isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».

Coriell houses the world's largest biorepository of cell cultures and offers medical researchers from around the world access to these patient cell lines for study of disease genes, use in screening -LSB-...]

In cultured human cells and in rabbits with implanted stents, Finn and colleagues showed that metformin augmented the effect of mTOR inhibitors on regrowth of the blood vessel lining.

Bertram JS, Janik P. Establishment of a cloned line of Lewis Lung Carcinoma cells adapted to cell culture.

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

We concluded that an expanded Revive & Restore, in partnership with biotech organizations, will be able to offer services that include Genetic Insight (DNA sequencing and analysis), Biobanking (tissue collection and cell culturing), Advanced Reproductive Services (cloning and germ - line transmission), and Genome Engineering (CRISPR, gene - drive, etc.) Applications include restoring genetic diversity, augmenting adaptation to climate change, conferring resistance to disease, and extirpating harmful invasive species.

Similarly, biopsies are obtained from many subjects and skin cells (fibroblasts) are isolated from these specimens, dedifferentiated to iPS lines, and reprogrammed and studied as neural and glial lines in culture.

Thirdly, the current, almost universal dependency of organoid culture on the use of Matrigel as a replacement for the function of the extracellular basement matrix in providing structural support and survival signals to the epithelial cells hampers clinical application, considering its origin from a mouse sarcoma cell line, its poorly defined composition and its mechanical rigidity after plating.

To address this problem, we developed a robust hepatocyte differentiation protocol — which promotes a highly synchronized differentiation pattern across multiple hiPS cell lines [1]-- and a novel maintenance medium, which enables the long - term culture of our hiPS cell - derived hepatocytes.

Glial cell line - derived neurotrophic factor promotes the survival of early postnatal spinal motor neurons in the lateral and medial motor columns in slice culture.

Cellartis human iPS cells from the ChiPSC22 line (Figure 2) and the ChiPSC18 line (Figures 3 — 7 and Table I) were grown in the Cellartis DEF ‑ CS 500 Culture System before editing.

To generate your own edited clonal hiPS cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media Cell Cloning DEF - CS Culture Media Kit:

All the hES and human induced pluripotent stem cell lines were confirmed to be genetically stable and pluripotent in in vivo and in vitro assays after extensive culturing on the new substrata.

Our study shows that selecting the appropriate cell line, growing the cells as 3 - D aggregates and infecting them when they are fully differentiated is key for successful in vitro cell culturing of human noroviruses.»