Sentences with phrase «cultured cell model»

Not exact matches

«The fact that a [cell] culture expresses most genes present in the brain says nothing about its appropriateness as a disease model,» says Knoblich.

This study identifies myomerger as a fundmentally required protein for muscle development using cell culture and laboratory mouse models.

Researchers then tested cell cultures and mouse models by using a gene editing process called CRISPR - Cas9 to demonstrate how the presence or absence of myomaker and myomerger — both individually and in unison — affect cell fusion and muscle formation.

With our human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»

A few drugs have shown promise in cell culture or animal models, but clinical trials in humans are time consuming because of the slow onset and progression of the disorder's clinical symptoms.

Often times we'll use an animal model or cell culture model to test a bioactive to see if it has efficacy.

However, this coupling does not exist in cell culture models with exogenously expressed c - MYC — the type of systems that are mainly used to investigate glutamine addiction in cancer.

This was observed in human ovarian cancer cells grown in culture, and then in mouse models of the disease.

Besides displaying more natural signaling patterns, 3D cultures allow researchers to mix cell types in ways that mimic their interactions in living tissue, providing more accurate models of normal physiology and pathogenesis.

To unravel the complex journey of the body's GPCRs, scientists used biophysical tools, including fluorescent biosensors; biochemical tools, such as cell signaling in neuronal cultures; as well as computational models.

The journal's home page explains that translational medicine «builds on basic research advances — studies of biological processes using cell cultures, for example, or animal models — and uses them to develop new therapies or medical procedures.»

Studies of IBD are typically performed using cell culture experiments or animal models, which don't mimic the precise conditions that occur in the gut of human patients.

«We compared the ability of RSV and parainfluenza virus (PIV3)-- another common virus in children that causes much less severe airway disease — to infect and cause inflammatory responses in a cell culture model of human epithelial cells, which compose the lining of the lung airway.

One postdoc presents data on her efforts to develop an organoid model for small - cell lung cancer; another reports progress on culturing hormone - secreting organoids from human gut tissue.

In 2016, the U.S. National Cancer Institute launched a scheme to develop more than 1000 cell culture models, including organoids, for researchers around the world to use, together with Cancer Research UK in London, the Wellcome Trust Sanger Institute in Hinxton, U.K., and HUB.

The test procedure is performed on an in vitro skin model built at Fraunhofer IGB from human skin cells in special culture dishes.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

Next, the team used other mouse models in which part of the lung was removed and single cell culture to study the plasticity of cell types during lung regrowth.

She uses lab models, including cell cultures and mice, to study these diseases.

Existing cell culture models were not very realistic, Tuveson says, and creating genetically engineered mice took up to a year, compared with up to 3 weeks for pancreatic cancer organoids.

«To do that, we went back to the cell culture model to ask whether or not the antibody basically prevents the uptake of misfolded α - syn.»

He is also developing a robust and comprehensive panel of 3 - D cell culture models from patient - derived primary cells that can be used to characterize different disease phenotypes and investigate the chemo - response of cells to novel or known drugs.

«We have provided a scalable and versatile 3D cancer model that shows a greater resemblance to natural cancer than 2D cultured cancer cells,» says the lead author, Professor Wei Sun of Tsinghua University in China and Drexel University in the United States.

The current work, she explains, uses antibodies that were generated and characterized at CNDR previously to see if they would reduce the pathology both in cell culture and in animal models.

We have been using cell culture models and zebrafish as an assay to determine the effects of BMP on signaling, but we also want to be able to understand what the effect of the mutation is in the tissue and organ systems.

Critically, despite the detection of enhanced Zika infection in cell culture models using antibodies from macaques previously exposed to either yellow fever or dengue and then exposed to Zika, there were no signs of enhanced Zika infection seen in these macaques themselves.

Cell culture and mouse models of prostate cancer confirmed the mathematical model predictions.

Using tissue culture models of cartilage and the meniscus, Levenston's team stresses the cells and tissues and studies what happens to them structurally and biochemically — for example, whether different genes are expressed, whether metabolism changes, or whether different types of cartilage cells respond differently to the same stress.

«This connection can not be identified in animals or in two - dimensional cell culture models.»

When he genetically disabled or chemically modified the HSPGs in cell cultures and in a mouse model, clumps of tau could not enter cells, thus inhibiting the spread of misfolded tau from cell to cell.

Fiumera works in vivo with fruit flies while Mahler works in vitro using a 3 - D cell - culture model of the gastrointestinal (GI) tract to understand how ingesting nanoparticles influences glucose processing and the gut microbiome.

The study, which used both cell cultures and mouse models, analysed how quickly Amyloid beta proteins, which are thought to be a likely cause of Alzheimer's disease, entered the retina and how they damaged it.

The scientists» model looks at the EGFR signaling cascade to investigate crosstalk between EFGR signaling and EMT in cell culture models of human breast epithelium.

A new study shows coibamide A has potent anti-cancer activity in mice and cell cultures that model brain tumors and triple negative breast cancer, two of the most aggressive and difficult - to - treat types of cancer.

The test drug slowed the growth of kidney cysts in two mouse models and in cell cultures of human kidney cysts, the study showed.

To better understand this sensitivity, a cross-disciplinary team of researchers led by Yale created models of the mutation in cell cultures.

«Indeed, this model of cell culture may reproduce what happens under physiological conditions.»

Also, in both cell culture and a mouse model of the condition, blocking the excessive splintering reduced production of reactive oxygen species, a potentially harmful byproduct of metabolism, as well as cell death.

The research team then tested RMC - 1 in cultured human airway epithelial cells and mouse models of asthma (via injection into the animals» peritoneal cavities).

To show that the Cal - Light technique is effective, Dr. Kwon's group tested it first in cell culture, and then in vivo in a mouse model.

Those doses were chosen in analogy with the prion field, in which it is very common to observe that 1 % and 0.1 % tissue homogenate containing prion protein aggregates is able to induce pathological changes both in cell culture experiments and in in vivo animal models.

The cellular model of the airway mucosa could provide insight into biological and pathophysiological effects that conventional cell cultures or animal models do not capture, and help lead to the development of new therapeutics.

In recent years cultured Drosophila S2 cells have been used extensively as a model system to study infection and immune responses.

Several tests in cultured cells indicated that ganciclovir would cause the death of HSV - TK - expressing stem cells, and testing in the mouse model confirmed that administration of the drug after successful treatment of brain metastases successfully eliminated therapeutic stem cells that also expressed HSV - TK.

These models include in vitro experiments with purified recombinant or synthetic proteins, cell culture experiments, and infectivity studies with Tg mice expressing the appropriate form of the protein.

Learn the basics about how to incorporate this model system into your research, including how it compares to existing 3 - D cell culture and organoid models.

Now, researchers at Harvard and Massachusetts General Hospital (MGH) and their colleagues have shown that amyloid - β can protect against yeast and bacterial infections in two animal models, as well as in cultured human cells.

Establish infrastructure to test repurposed drugs in cell culture and animal models for potency, pharmacokinetic (PK), and pharmacodynamic (PD) studies.

Mouse embryonic stem cells stained for key pluripotency markersGRAZIANO MARTELLO Combining computer science algorithms with biological data has enabled researchers to create a model of the minimal number of factors necessary for mouse embryonic stem cells (ESCs) to self - renew in culture.

This video provides an overview of MimEX ™ Tissue Model Systems, a next - generation 3 - dimensional (3 - D) cell culture platform for the generation of ex vivo epithelial - derived organ tissue.