Sentences with phrase «cultured cell samples»

Execution of DNA extraction using the Promega genomic DNA purification system on cultured cell samples.

Not exact matches

Rather than obtaining meat from animals raised on environmentally destructive factory farms and slaughtered in filthy slaughterhouses, clean meat is produced by taking a small sample of animal cells and replicating them in a culture outside of the animal.

Rather than obtaining meat from animals raised on environmentally destructive factory farms and slaughtered in filthy slaughterhouses, clean meat is produced by taking a small sample of animal cells and replicating them in a culture outside of the animal.

New developments include reaction systems for catalysis research, sampling from reactors, systems for high - throughput applications in synthesis, the handling of microorganisms or cell - culturing procedures, and more.

A tissue slice, a tumor biopsy, or a sample of a bacterial culture yields a sequence representing the average of all of the cells within it, even though researchers know there can be tremendous variation between those cells.

Previously, high - resolution live imaging has been done with cells cultured on glass slides, which flattens samples.

If it proves positive, the researchers will mix another portion of the sample with cultured bat and monkey cells to test for active virus.

Hsiao and colleagues carefully gathered skin samples from donors, and then grew the skin cells in culture before converting them into iPS cells using the methods created by Yamanaka.

Dr. Islam saw this phenomenon occur in cell culture and in some samples from patients with head and neck cancers.

The new method has numerous advantages: researchers can sample individual cells of a tissue culture directly in the petri dish.

They confirmed low levels of miR - 184 expression in human glioma tissue samples and cultured cell lines as well as an increase in the expression of SND1 compared to normal brain tissue.

A microreactor developed as part of an EU - funded collaborative research project enables cultured liver cells to be used as test samples.

Another option is to take a skin sample, culture cells called fibroblasts and freeze them.

Researchers rushed to the building the morning after the quake to wade through chemical - laced water and salvage cell cultures, enzymes, antibodies, and other frozen samples and reagents, bringing some home to their kitchen freezers.

They then conducted biochemical analyses to identify neuroligin - 3, confirm that the protein could stimulate tumor growth in cultured samples of several kinds of human high - grade gliomas and study which signals the protein uses within glioma cells to promote their growth.

The scientists say their gadget provides a quicker diagnosis that today's typical method, in which a cell sample is taken and cultured in the lab to look for the presence of the Candida albicans fungus.

The structure and composition of the erionite from both regions is practically identical, and samples from each place cause mesothelioma - associated changes and inflammation in cell culture and mouse lungs.

To confirm that the missing mutations were important for generating an immune response, the researchers cultured a subset of the neoantigen protein fragments containing the tumor mutations with immune cells taken from three patients» blood samples.

The team conducted additional experiments in which mouse breast tumors were sampled to generate so - called organoids — spheres of cells that grow in three - dimensional culture.

They diluted the microbial sample such that they could add a single cell per culture well filled with their new media via a technique called dilution - to - extinction.

Institut Pasteur scientists are now testing additional samples from the 24 patients and waiting for cell culture results.

The team grew a weakened strain of dengue in vats of cultured mosquito cells, froze the samples, and examined the virus particles under an electron microscope.

The team collected two small skin samples from each, and then cultured the cells in the lab and inserted the gene that makes fireflies glow into the gene that regulates the cells» clock.

Scientists at the University of Luxembourg have succeeded in turning human stem cells derived from skin samples into tiny, 3 - D, brain - like cultures that behave very similarly to cells in the human midbrain.

But the scientists can't be certain these cells are perfect matches for native human motor neurons since it's difficult to obtain samples of cultured motor neurons from adult individuals.

After achieving 80 — 90 % confluency, cell culture media were changed to maintenance media with 2 % FBS and were inoculated with 200 µL of clinical sample or 100 µL of passaged viral supernatant.

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

This facility is in charge of producing cell cultures from patient samples for both diagnosis (prenatal diagnosis; Ulrich and Betlem myopathies; seronegative myasthenia) and biobanking purposes.

In order to test whether the particles could change the behavior of cells, the team injected a sample onto cultured rat dorsal root ganglia neurons, which are found in the peripheral nervous system.

To measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the pH indicator phenol red.

For some applications, a qPCR wil... Keywords: Amplification, Cancer, Capillary electrophoresis, Cell culture, Degradations, Detection methods, Electrophoresis, Gene expression, Growth factors, Indicators, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sample preparations, Transcription

U-M's internationally renowned Translational Neuro - Oncology Laboratory generates novel primary cell cultures from pediatric and adult glioma tissue samples to study mechanisms of glioma growth and targeted treatment response.

The team conducted tests on three different samples of cells aged in different ways: cells taken from aged donors, cells aged in culture in the lab, and those isolated from patients suffering from Hutchinson - Gilford progeria syndrome, a rare genetic disorder that ages sufferers at an accelerated rate.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

Such stem cells are «immortal» in the lab, meaning there is no need for new cell cultures or re-harvesting from additional tissue samples.

Users can provide frozen slices of tissue, entire frozen tissue sample ready to be sliced, or cultures of cells.

Samples should be provided for in sufficiently concentrated stock and be compatible with cell culture (i.e. in vitro analysis).

By using a common cell culture step, it is possible for the pathogens in a sample to multiply before the test, which allows for detection of a single original cell.

Cheng and his colleagues cultured lung spheroid cells from these tiny tissue samples until they were numerous enough — in the tens of millions — to be delivered therapeutically.

Frozen total RNA from any human sample and / or human derived cell - cultures.

Inset corresponds to high resolution image showing d lattices; (B) Energy dispersive X-ray analysis showing Ag but no impurities; (C) zeta potential of different prepared samples; (D) DLS of samples in cell culture medium showing well - dispersed nanoparticles.

Depending on which lung lobe is selected, the doctor will then «wash» the lungs with sterile saline and then suction the wash fluid back to obtain cells for cytology and a sample for culture.

The most accurate method for diagnosing ringworm in dogs is by fungal culture of a sample of hair or skin cells.

Seroneutralisation on puppy kidney cell cultures (collection in dry tube (no anticoagulant) of two samples of the mother's serum at 10 - 15 day interval, the first sample being collected in the days following abortion or stillbirths).

She collects DNA (blood draw) or relevant tissue samples at surgical biopsy time or at definitive surgical correction, and processes the sample (prepares for RNA extraction, flash freeze or cell culture).