Sentences with phrase «cultured cells derived»

They turned to cultured cells derived from human lung tumours.

Using cultured cells derived from human tumors of the breast and prostate gland, they confirmed that the IL6R / STAT3 / miR -34 a feedback loop is also activated in other tumor types.

When the Stanford team measured pyrophosphate levels in cultured cells derived from ank and normal mice, they found that the chemical accumulated in cells from the ank mice but decreased in the culture medium.

Not exact matches

Since the algae reproduces by cell division about once per day in the laboratory, numerous genetically identical cultures could be derived from the isolate.

Existing gels for cell culture are often animal derived, which can affect the reproducibility of results or, if synthetic, require coatings or coupling of cell - adhesive ligands.

In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagen.

While there, he worked with cell cultures derived from human fetal tissue.

They found that indeed, they do, and that stimulating these cells led them to kill cells infected with HIV - 1 derived from latently infected cells, both in culture and in mice engineered to have a human immune system.

A Freiburg - based research group has developed a microfluidic chip where more than one hundred apidose - derived adult stem cell cultures can grow and divide.

In BRIC 17 - 1, cell cultures derived from thale cress plants are grown in Petri dishes and later examined to determine which genes are involved in certain cellular changes.

He is also developing a robust and comprehensive panel of 3 - D cell culture models from patient - derived primary cells that can be used to characterize different disease phenotypes and investigate the chemo - response of cells to novel or known drugs.

She added bacterial cells to a culture of a widely used mammalian cell line derived from the ovaries of Chinese hamsters.

Grima also observed this same clumping of Huntingtin protein with RanGAP1 and nuclear pore proteins to the wrong place in the cell in brain tissue and cultured brain cells derived from deceased patients with Huntington's disease.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channel.

When the Cornell team cultured human breast cancer cells on matrix deposited by fat - derived cells from obese mice, the cancer cells grew faster than they did on the matrix of cells from slimmer mice.

Further testing of this culture method on kidney progenitor cells derived from human iPS cells resulted in four times the total amount of cells over 8 days.

Testing many variants, the team eventually found an artificial receptor design that worked well in cell culture, enabling host T cells to efficiently destroy cells producing antibodies to desmoglein, including those derived from PV patients.

Researchers used cultured stem cells derived from their feline patients in the hope of reducing inflammation and promoting tissue regeneration.

Added to patient - derived cells in culture, rapamycin enabled the development of more Purkinje precursor cells, improved the functioning of their synapses and increased their tendency to fire.

Although a clause in the law that funds NIH prevents the agency from funding research that would harm or destroy an embryo, a lawyer at the Department of Health and Human Services ruled in 1999 that because stem cells — which can grow ad infinitum in culture — are not themselves embryos, the NIH could fund work with cells that were derived by privately funded researchers or researchers overseas.

In this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.»

Scientists at the University of Luxembourg have succeeded in turning human stem cells derived from skin samples into tiny, 3 - D, brain - like cultures that behave very similarly to cells in the human midbrain.

When BMP protein was added for three days in human pluripotent stem cell - derived gut tube cultures, it induced a posterior HOX code.

The percentage of cells expressing both E-selectin and VCAM - 1 (or DiI - Ac - LDL for bone - derived cultures) after 5 h of TNF - α stimulation in the second series of selection increased significantly to reach values of 30 — 40 % for most cultures.

Phase - contrast images of organ - derived endothelial cells (37 °C culture conditions) that had undergone two successive selection processes by flow cytometry.

However, chondrogenesis assays in chick micromass cultures showed that enhanced signaling by the mutant R206H ACVR1 can be partially inhibited by the BMP antagonist Noggin, suggesting that in addition to BMP - independent activity by the R206H ACVR1 receptor, this mutant receptor is also responsive to BMP, consistent with previous BMP signaling pathway studies that used cells derived from FOP patients (48 — 50).

The second step will be to optimize culture conditions for the commitment of the iPS / hES cell - derived retinal precursors towards the photoreceptor lineage.

This video provides an overview of MimEX ™ Tissue Model Systems, a next - generation 3 - dimensional (3 - D) cell culture platform for the generation of ex vivo epithelial - derived organ tissue.

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

To address this problem, we developed a robust hepatocyte differentiation protocol — which promotes a highly synchronized differentiation pattern across multiple hiPS cell lines [1]-- and a novel maintenance medium, which enables the long - term culture of our hiPS cell - derived hepatocytes.

Glial cell line - derived neurotrophic factor promotes the survival of early postnatal spinal motor neurons in the lateral and medial motor columns in slice culture.

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using a novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Differential osteogenicity of multiple donor - derived human mesenchymal stem cells and osteoblasts in monolayer, scaffold - based 3D culture and in vivo.

Studying the drug - responsiveness of breast cancer cells cultured within human osteoblast - derived matrices.

Characterization and Culturing of Adipose - Derived Precursor Cells.

Suspension culture of hepatocyte - derived reporter cells in presence of albumin to form stable three - dimensional spheroids.

To ensure that any pathology developed in the cultured neurons could be attributed solely to the genetic defect, the researchers also derived control neurons from iPS cells in which the mutation had been corrected.

Delaney et al. [4] who have shown that cord blood - derived CD34 + cells cultured in StemSpan ™ medium with cytokines, on plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.

The study used three cell types: human motor neurons derived from induced pluripotent stem cells, rat cortical cultures, and human breast cancer cells.

To highlight the potentially important role of 3D culture conditions, the authors compared 2D monolayers of hepatocyte progenitors, fetal liver stromal cells, or adult bone marrow - derived stromal progenitor cells to their 3D counterparts cultured on liver extracellular matrix (3D - ECM) constructs.

The portfolio includes U.S. and foreign patents and applications directed to culture, expansion and scale - up of pluripotent stem cells and cell types derived therefrom, various differentiation processes for production of mesendoderm, definitive endoderm, PEC - 01, and endocrine precursors, as well as claims directed to the Company's Encaptra ® macro-encapsulation device platform.

Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.

For this purpose, at least 30 different cell lines derived from biopsies (HNSCC — head and neck cancer — patients) wil be collected and grown in culture to esstablish cell lines.

Bone marrow cells cultured with conditioned medium derived from ABL1 / ABL2 knockdown 1833 and SKBR3 breast cancer cells had decreased numbers of TRAP + cells compared to the control groups (Fig. 5, B and C, and fig.

Defining Adipose Tissue - Derived Stem Cells in Tissue and in Culture (June, 2010) in Histology Histopathology.

These compounds also corrected SMN2 RNA splicing and increased SMN protein levels in cell cultures obtained from SMA patients, including stem cell - derived motor neurons.

Derivation of completely cell culture - derived mice from early - passage embryonic stem cells.

Cells were cultured for 10 d following plating and 12 wells derived from each fraction were subjected to flow cytometry.

The United Kingdom is developing a stem cell bank that would make a variety of characterized and newly derived stem cell cultures available to researchers.