Not exact matches
What it
does: Scientists have nicknamed mycoplasmas the «crabgrass» of
cell cultures because they're particularly tricky to detect, diagnose, and eradicate.
Cultured meat producers still have to source the first set of
cells from an animal — even if it's just a small biopsy that doesn't require slaughter.
If Christians believe that the church and the worship it offers to God ought in some ways to counter the norms and practices of the surrounding
culture, then what
does it mean that after spending so much of our time each week in front of computer monitors,
cell phones, and sports bar TVs, we come to church on Sunday and happily position ourselves in front of the biggest screen of all?
While some still object to
cells being taken from animals and used by scientists to grow clean meat in laboratories — and some just don't like the idea of eating a «
cultured» steak created by men and women in white coats — others see the lab - grown meat revolution as key to solving the environmental crisis linked to meat eating.
This allowed them to track changes in calcium ion concentrations in the
cells around wounds in living tissue (as opposed to the
cell cultures used in many previous wound response studies) and to
do so with an unprecedented, millisecond precision.
Or
did you think that
cell culture would grow healthy without mommy feeding it regularly?
Yet Pereira - Smith, who still pops into the lab periodically to
do her own
cell culture work, was always available to discuss results and offer encouragement.
Bioengineers at the University of California, San Diego have proven that when it comes to guiding stem
cells into a specific
cell type, the stiffness of the extracellular matrix used to
culture them really
does matter.
The team found several hundred that might
do so and restore the protein to its functional shape; more than 30
did the job when tested in
cell culture, although they don't know whether they attached to p53 in the pocket.
Yeast
cultures normally
do not exceed 100 million to 200 million
cells per millilitre.
Milk glands, moreover,
do not need the constant coddling required for
cell cultures.
Previously, high - resolution live imaging has been
done with
cells cultured on glass slides, which flattens samples.
When
cells that produced IL - 21 were put in
culture with synovial fibroblasts (which are the main contributors to joint inflammation in rheumatoid arthritis), they induced the production of proinflammatory cytokines by these synovial fibroblasts, and
cells that
do not produce IL - 21,
did not demonstrate this same outcome.
«The argument is whether these
cells will
do it in the body normally, or is this a feature of the
cells being
cultured,» Telfer says.
However, this coupling
does not exist in
cell culture models with exogenously expressed c - MYC — the type of systems that are mainly used to investigate glutamine addiction in cancer.
The
cell cultures still
did not prove whether optogenetics would apply to brain
cells inside living, freely moving mammals, however.
«The concentrations of anabasine tested here are 100-fold lower than those required to inhibit growth of parasite
cell cultures,» Palmer - Young says, «and we
did not find any evidence for variation in anabasine sensitivity across parasite strains.
Although no tests have yet been
done on real wounds, experiments on
cultured human skin
cells have been encouraging, he says.
They found that when IGF - 1 was added to the
cultured blood
cells there was increased lithium sensitivity only in the blood
cells of those bipolar disorder patients who
did not respond to lithium therapy.
We were able to use pharmaceuticals to induce
cell self - eating effects and stimulate dedifferentiation though not as effectively as 3D
culture, so we need to
do more work on this.
Three types of colon cancer
cells grew faster, producing about 50 to 60 percent more
cells within 24 hours, with the bacteria than they
did when
cultured with no bacteria or with a harmless, milk - fermenting bacterium called Lactococcus lactis.
«In a given week I'll
do cell culture, protein chemistry, molecular biology, microscopy, and structural biology on the computer,» adds Luhrs.
I found that
culturing cardiac valve
cells on synthetic hydrogels preserves their normal properties better than the traditional plastic plates
do, and I discovered a signaling pathway connecting the stiffness of the supporting scaffold to the
cells» cytoskeletal structure.
2 - D
cell -
culture and mouse experiments also provided key evidence of the virus's modus operandi; although the rodent brain doesn't harbor the full contingent of human neural stem
cells, it has blood vessels and immune - system components that organoids lack.
But the transition from brain -
cell cultures to the brains of intact animals
does not seem to bode well for astronaut resilience.
They noticed that while the dermal papilla
cells from mice naturally formed large clumps in
culture, the human
cells didn't.
They found that indeed, they
do, and that stimulating these
cells led them to kill
cells infected with HIV - 1 derived from latently infected
cells, both in
culture and in mice engineered to have a human immune system.
Studies of IBD are typically performed using
cell culture experiments or animal models, which don't mimic the precise conditions that occur in the gut of human patients.
In
cells grown on flat
culture dishes, the expression of thousands of genes didn't match up with their normal patterns, explaining why the
cells from those dishes had been unable to generate new hair follicles.
Cantley's lab and collaborators found that large doses of vitamin C
did indeed kill
cultured colon cancer
cells with BRAF or KRAS mutations by raising free radical levels, which in turn inactivate an enzyme needed to metabolize glucose, depriving the
cells of energy.
«Five years later,» Daley said, «I still don't know of any laboratory that has been able to
culture those
cells.
«To
do that, we went back to the
cell culture model to ask whether or not the antibody basically prevents the uptake of misfolded α - syn.»
The treatment
did a lot to prevent
cell migration, a key mechanism in metastasis, in experiments on laboratory
cultures (in vitro) of cancerous human
cells.
They found that the infected
cultures responded to the drugs in the same way that liver
cells inside the body are known to
do.
To
do this, they incorporated the gene sequence of each receptor into
cultured cells and then probed the
cells to determine if they were activated by one or more of 25 different bitter - tasting chemicals.
This is the reason why haploid
cell cultures do not thrive.
Cell culture systems used today also
do not faithfully represent the features of human neurons.»
Using human T
cells in
culture as well as mice lacking one of these genes, YY1, they found that, indeed, the two proteins
did help move Xist back to the inactive X chromosome in activated lymphocytes.
Traditionally,
cell culture in the lab has been
done in petri dishes and on other flat surfaces.
«
Do those
cells actually exist in the body, or are they created from the
culture conditions?»
One major roadblock, Miglietta told me, is that most of the work on transcription factors has been
done on
cells grown in a
culture dish.
Investigations into human brain development using human
cells in the
culture dish have so far been very limited: the
cells in the dish grow flat, so they
do not display any three - dimensional structure.
When the Cornell team
cultured human breast cancer
cells on matrix deposited by fat - derived
cells from obese mice, the cancer
cells grew faster than they
did on the matrix of
cells from slimmer mice.
If the ad refers to
cell culture experience,
do not spend that important middle paragraph describing your experience with E. coli.
Normal
cells often have proved impractical because they can only divide a limited number of times in
culture, and once returned to the body they're often too old to
do much good.
When Patapoutian and his colleagues added each sulfur compound individually to
cultured cells, only allicin triggered a fast, intense response like the raw extract
did.
Previous research showed that COX - 2 inhibitors cut viral production in half in
cultured cells, but how they
did so was unclear.
Only in 2004
did scientists finally succeed in growing such
cells in
culture from mice.
«Our
cell cultures open new doors to brain research,» says Prof. Dr. Jens Schwamborn, in whose LCSB research group Developmental & Cellular Biology the research work was
done.
Another, previously unknown gene — when the investigators worked out its protein's structure and studied it in tissue
culture — proved to
do just the opposite: The protein helped the schizont leave the infected blood
cell.