To survey exactly which bacteria induced TH1
cells in the gut of the TH1 induced mice the researchers isolated and
cultured the microbes from the feces, and then separately
inoculated each microbe to germ - free mice.
After achieving 80 — 90 % confluency,
cell culture media were changed to maintenance media with 2 % FBS and were
inoculated with 200 µL of clinical sample or 100 µL of passaged viral supernatant.