Sentences with phrase «cultured cells suggested»

Additional experiments with cultured cells suggested that the mutation interferes with one of the enzymes that breaks down the APP protein and causes a 40 % reduction in β amyloid formation, Stefansson and colleagues report online today in Nature.

Not exact matches

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

A host of cell culture and animal data suggests they should work.

The process was so effective that 20 days later, the cell culture showed no evidence of any revival, suggesting that the tumour cells had been destroyed.

They also suggest it may soon be possible to mature the cells in a laboratory dish simply by adding a cocktail of microRNAs to the cell culture.

The new research, which studied the immortalization process using genome - engineered cells in culture and also tracked skin cells as they progressed from a mole into a malignant melanoma, suggests that telomerase plays a more complex role in cancer.

Results suggest that cells grown in the device exhibit more natural behaviors than when grown in traditional culturing methods, and the filtration by the glomerulus is necessary for healthy cell function.

That's an important lesson, Tolar says; it suggests that future attempts to correct genetic skin diseases should focus on culture conditions that nourish these stem cells, and potentially even target them for modification.

Studies by Bausch and others have also detected live Ebola virus in sexual fluids that can successfully grow in cell culture, suggesting it could also lead to infections in other individuals.

«Our results suggest that megakaryocytes might be used clinically to facilitate adult stem cell regeneration and to expand cultured cells for adult stem cell transplants,» says Meng Zhao, Ph.D., a postdoctoral fellow at Stowers and lead author on the study.

In cultured cells, drugs that inhibit Cdk1 prevented HIF - 1alpha levels from falling and restored its ability to halt cell division, suggesting they may be effective treatments for certain cancers.

The researchers found that overproducing the microRNAs in cultured cells cut down the concentration of E2F1, a protein involved in a cancer growth pathway, suggesting that, in healthy cells, the microRNAs keep cell growth in check.

The test could be made, he suggests, by exposing cultured muscle cells to adrenaline at temperatures between 20 °C and 40 °C.

However, chondrogenesis assays in chick micromass cultures showed that enhanced signaling by the mutant R206H ACVR1 can be partially inhibited by the BMP antagonist Noggin, suggesting that in addition to BMP - independent activity by the R206H ACVR1 receptor, this mutant receptor is also responsive to BMP, consistent with previous BMP signaling pathway studies that used cells derived from FOP patients (48 — 50).

In contrast, in cultures treated with X4 - ZFNs viral titers steadily decreased after peak viremia while cell growth remained exponential suggesting there was not significant viral production (data not shown).

Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ cell cultures with TNF - α (10 ng / ml) and targeting cells expressing the endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.

We then prepared cultures from this tissue, but we observed only very few GFAP or S100β positive astrocytes, while the majority of cells were negative for all glial and neuron markers tested, suggesting that these cells may be non-neural.

Researchers have discovered a gene in zebrafish so powerful it can be used to redirect the fate of cells in the developing embryo to become beating heart cells, suggesting that a similar gene in humans could be used to generate heart cells in culture for transplant in ailing people.

This research has been suggested before using cell cultures and in artificially aged mice, but this is the first time that a naturally - aging model has been used.

However, a recent published review of basic science journals, including studies of cells in culture from high impact cardiovascular journals, and of basic and clinical scientific literature, suggests that sex of experimental material is not consistently reported.

Unfortunately, inclusion of the 2i in our culture system resulted in massive death of irradiated MEF cells plated at a relatively low density, suggesting that the 2i strategy might not be compatible with irradiated MEF cells.

Assessment of hASC - CM composition found high expression of various human growth factors (IL ‐ 6, 8, 12, eotaxin, IP10, MCP ‐ 1, VEGF, and TIMP ‐ 1) in the supernatant following the co-culture of hASCs with islet cells, while IP10, eotaxin, VEGF, and TIMP ‐ 1 became increased with time during islet co ‐ culture, suggesting the presence of paracrine cross ‐ talk between islets and hASCs.

Cells with similar characteristics accumulate during normal aging as well as in younger persons infected with human immunodeficiency virus, suggesting that the process of replicative senescence is not an artifact of cell culture but is also occurring in vivo.

Recent observations suggest that there may be lineage - specific markers expressed in sub-populations of ES cell cultures.

The observation that SCP1 was expressed in EBs even without extrinsic factors (Figure 5B) may suggest that the components of the culture medium are also one of important factors to effectively induce germ cell differentiation, especially meiotic progression in vitro.

ES cell cultures also express low levels of Gata4 and Gata6, suggesting the presence of either background levels of PrEn gene expression or basal levels of PrEn differentiation [25], [27].

It was recently suggested that Hes1 expression can cycle in ES cell culture [13], although the link between this oscillation, low - level gene expression, and developmental bias is not clear.

Thus it has been suggested that ES cell cultures can be split based on Rex1 and Oct4 [9], into Rex1, Oct4 positive ICM, and Rex1 negative Oct4 positive PrEc.

The existence of a paracrine inter-dependent equilibrium would suggest that the culture conditions have selected for the stable coexistence of mutually dependent and metastable cell types that only transiently exist in vivo.

VASA was detected earlier in cells cultured in testicular cell - conditioned medium than in those cultured in non-conditioned medium or in ovarian cell - conditioned medium, suggesting that the initiation of germ cell differentiation in testicular cell - conditioned medium was faster than that in ovarian cell - conditioned medium.

No significant differences were seen between the normoxic and hypoxic anoikis - resistant populations collected from MCF7 cells and a single significant gene change (PIP) was seen in T47D (Table 1) suggesting that the CSC - enriched population remains virtually unchanged following hypoxic culture and the increase in MFC, HFC, and tumor initiating cells is, therefore, due to expansion of the population, perhaps by increased symmetric self - renewal of the CSC or de-differentiation of early progenitor cells, rather than simply the acquisition of anoikis resistance in non-CSC.

Therefore, twelve day old, DMSO - differentiated, Ara - C treated P19 cultures were incubated with the proteasome inhibitors MG - 132 and lactacystin for four hours; this was sufficient to rescue Oct4 protein expression (Figure 1D), suggesting that these cells do indeed synthesize new Oct4 protein.

These results support data suggesting that excess HS oligosaccharides impair function of cellular adhesion molecules and disrupt normal polarization and orientation of cultured MPSIIIB mouse astrocytes or neural stem cells [52].

As for the EWGâ $ «referenced studies suggesting the dangers of cosmetic ingredients, Bailey says, «Many of these studies involve cell cultures where you take cells out of the body and you expose them to very high concentrations of a material.»

Cell culture assays suggest chasteberry components have anti-inflammatory and anti-tumor activities.

Painted in formal and spiritual unity, the diamonds, rectangles, and zigzags that comprise these sequences suggest foundational real - world materials: bricks, religious icons, single - celled organisms — things of which history and culture are formed.

Our study also suggests that cellular responses to future ocean conditions are not likely to be straightforward, as shifts in calcification may have less to do with calcification - related genes and more to do with intracellular regulatory processes in cells grown under continuous culture for many hundreds of generations.