Sentences with phrase «cultured cells with»

Total RNA was extracted from cultured cells with RNeasy Mini kit (Qiagen), with the use of QIAshredder spin column for homogenization and an on - column DNase digestion.

Incubating cultured cells with RK - 33 had a similar effect, knocking down DDX3 expression in cells that highly express this protein and hampering their ability to multiply.

In this study, Nicchitta and his colleagues treated tissue culture cells with a stress - inducing agent called thapsigargin.

Not exact matches

Post, of MosaMeat, who created the first hamburger from cultured animal cells in 2013 with backing from Google billionaire Brin, believes some of his competitors have set unrealistic timelines to market in part because that's what tech investors want to hear.

The company debuted its first synthetic meatball in 2016 and followed up with the world's first cell - cultured chicken and duck earlier this year.

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

Heretofore, this earth has witnessed the emergence of single - celled living organisms, the growth of multicelled plant organisms, the advent of animals with centralized nervous systems making self - directed activity possible, and the flowering of humanity with its far - flung culture.

Juiced Curly Kale Kills Melanoma Cancer Cells In Culture Please share this information with family and friends.

The researchers experimented with inducing oxidative stress in a human cell line culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme in protecting the cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the liver.

EGFR / WT and KO cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were lysed and cox - 2 mRNA levels were analyzed by RT - PCR.

The study, «Polarity of varicosity initiation in central neuron mechanosensation,» which will be published June 12 in The Journal of Cell Biology, observes the swelling process in live cultured neurons and could lead to new ways of limiting the symptoms associated with concussive brain injuries.

Visually, she is filming and analyzing time - lapse images of human embryos in the incubator and has been able to correlate various parameters of how cells divide with the probability that the embryos will make it to a full blastocyst stage by day 5 - 6 of culture.

3M Delivers Early Purification with New Hybrid Purifiers, Single - use functionalised media provides scalable purification performance in cell culture

This allowed them to track changes in calcium ion concentrations in the cells around wounds in living tissue (as opposed to the cell cultures used in many previous wound response studies) and to do so with an unprecedented, millisecond precision.

But when the researchers added higher concentrations of BX795 to cultured human corneal cells infected with HSV - 1, the infection was quickly cleared.

Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

This method allows the scientists to measure very subtle effects with very high confidence, while eliminating the effect of any genetic or epigenetic modifications and cell culture related variations that could occur during the experiment.

They found high levels of the regulatory T - cells in patients treated with post-transplant cyclophosphamide, and lab - cultured cells survived cyclophosphamide treatment.

In the journal Biomaterials, the team reports culturing cells to make extracellular matrix (ECM) of two types and five different alignments with the strength found in natural tissue and without using any artificial chemicals that could make it incompatible to implant.

Cyclic adenosine monophosphate (cAMP)- dependent protein kinase, labeled with fluorescein and rhodamine on the catalytic and regulatory subunits, respectively, was injected into Aplysia sensory neurons either in culture or in intact cell clusters.

For instance, to make a rod with collagen fibers aligned along its length (like a tendon) they cultured chondrocyte cells in a dog bone - shaped mold with loops on either end.

The trophoblasts along with blood vessel cells were added to small dextran beads that were then spun around in a container filled with cell culture fluid, creating shear stress and rotational forces to better mimic the environment at the maternal - fetal interface than static cell - culture systems.

Finally, in cell cultures and animals, overexpressing the hormone could produce something that looks like cancer, and that would shrink when treated with an IGF - 1R blocker.

Working in cell cultures and mice, researchers at Johns Hopkins have found that an experimental drug called fostamatinib combined with the chemotherapy drug paclitaxel may overcome ovarian cancer cells» resistance to paclitaxel.

The Wyss team believes the ability of the human gut - on - a-chip to culture the microbiome with human gut cells also holds promise for the field of precision medicine, where a patient's own cells and gut microbiota could one day be cultured inside a gut - on - a-chip for testing different therapies and identifying an individualized treatment strategy.

With our human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»

To find out, the researchers injected a cloned telomerase gene into cultured cells from retina, skin, and blood vessels, all of which are associated with degenerative, aging - related diseases.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

Now researchers at the University of Plymouth, working in partnership with pharmaceutical company AstraZeneca, have for the first time successfully cultured and maintained cells from the guts of rainbow trout, a recommended fish species for toxicological studies.

«If we win, the entire regulatory structure for autologous cell processing, with or without culture, will be rewritten such that any physician using good practices and treating patients responsibly can use stem cells as part of his or her medical practice,» he says.

They discovered three chemical structures with potent antiviral activity against Ebola virus in cell culture.

Also, researchers could carefully select which blood types to culture with each batch of stem cells, creating stockpiles of needed blood products for people with extremely rare blood types whose blood cell makeup makes it challenging to find good blood matches for transfusions because they would reject most other types of blood.

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

Upon expression in cultured cells, the new subunits yielded prominent, typical glutamate - and NMDA - activated currents only when they were in heteromeric configurations with NR1.

They then used fluorescent antibodies that bind specifically to the protein to trace the virus's interactions with various kinds of cultured cells.

One would culture thin sheets of meat, seeded by cells from a living animal, on a reusable polymer scaffold; the other would grow meat on small edible beads that stretch with changes in temperature.

One of the 12 audience members was Ben Chao, a chemical engineer with a Ph.D. — not at all qualified to make a judgment on Scott's abilities in the area of «Approaches to Developing Serum - Free Media Formulations for Mammalian Cells in Culture.»

Subsequent tests revealed that the treatment had reduced the visible cell population by a factor of 100 thousand, compared with an untreated cell culture, within the space of just 20 days.

Previously, high - resolution live imaging has been done with cells cultured on glass slides, which flattens samples.

«The required enzyme can be produced biotechnologically with the help of cell cultures,» says Dierks.

Although the bulk of the commercial manufacturing uses cultures of bacteria, such as Escherichia coli or Chinese hamster ovary cells, a few biotech companies are trying to produce therapeutic proteins in the milk of transgenic mammals (such as GTC Biotherapeutics, which is using goats; PPL Therapeutics, which is using sheep; and BioProtein Technologies, which is working with rabbits), transgenic chicken eggs (such as Avigenics or Vivalis), or even in transgenic crops (such as ProdiGene or Meristem Therapeutics); but it is early days for these «pharming» methods.

Confocal microscopy of human neural stem cell culture infected with Zika virus (red).

«But we have problems finding PhDs with expertise in the [manufacturing] processes of cell culture, fermentation, and purification,» he adds.

What's more, those increases lined up with the cell culture data.

If it proves positive, the researchers will mix another portion of the sample with cultured bat and monkey cells to test for active virus.

The bacterium produced an antibiotic, dubbed lugdunin, which killed a slew of superbugs in cell cultures and mice with no signs yet of growing resistance.

When cells that produced IL - 21 were put in culture with synovial fibroblasts (which are the main contributors to joint inflammation in rheumatoid arthritis), they induced the production of proinflammatory cytokines by these synovial fibroblasts, and cells that do not produce IL - 21, did not demonstrate this same outcome.

Follow - up tests in human cell cultures confirmed that MIR168a interferes with production of a cholesterol - clearing protein.

In studies with human fibroblasts that make up connective tissue, Boger's team tested whether NOD1 activity could affect CMV replication in cultures of cells grown in the lab.

However, this coupling does not exist in cell culture models with exogenously expressed c - MYC — the type of systems that are mainly used to investigate glutamine addiction in cancer.