For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h.
Human colon cancer cell line HT - 29 and prostate
cancer cell line PC3 were obtained from American Type
Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.
A flavorful component of cinnamon oil, called cinnamaldehyde, may help prevent
colon cancer, according to a tissue
culture study of
human colon cancer cells published in the May 2010 issue of the journal «Molecules.»