Sentences with phrase «cultured in a humidified»

Not exact matches

All cultures were maintained at 37 °C in humidified air containing 5 % CO2.

SH - SY5Y cells were cultured in Dulbecco's modified Eagle's medium nutrient mixture F - 12 (DMEM / F12; Sigma), supplemented with 10 % FBS (Sigma), 100 nM l - glutamine (Sigma), 100 U / ml penicillin (Sigma), 10 µg / ml streptomycin (Sigma), in a humidified atmosphere containing 5 % CO2 at 37 °C.

Flask cultures were kept in a humidified incubator in a 5 % CO2 atmosphere at 37 ° C. Growth medium was replaced every 72 hours for two weeks.

Cells were then plated in 24 - well tissue culture treated plates and incubated in a humidified 5 % CO2 incubator at 37 °C.