Sentences with phrase «cultured the cells for»

The team then cultured the cells for several months in a bath of growth - promoting proteins, trying to get the cells to reverse course and become more like stem cells.

«Our results suggest that megakaryocytes might be used clinically to facilitate adult stem cell regeneration and to expand cultured cells for adult stem cell transplants,» says Meng Zhao, Ph.D., a postdoctoral fellow at Stowers and lead author on the study.

C, primary breast cancer cells isolated from patients with advanced cancer were directly infected with KLF4 or control lentivirus, and the CSC population (CD24 − CD44 + ESA +) was measured by FACS after culturing the cells for 72 hours in low - attachment plates.

Not exact matches

Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs of the growth medium necessary for culturing the desired cells.

In fact, these dead starter culture cells and debris are an important food source for subsequent generations of microbes, referred to as non-starter lactic acid bacteria (NSLAB).

He has more than fifteen years» experience in the life science industry, including the product development, market development and commercialization of cells, scaffolds, cell reprogramming tools and cell culture media for regenerative medicine and bioprocessing applications.

EGFR / WT and KO cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -cells were cultured overnight and starved for 24 h. Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were treated with capsaicin (50 μmol / L) for 30 min and then TPA (20 ng / mL) for 2 h. Cells were lysed and cox - 2 mRNA levels were analyzed by RT -Cells were lysed and cox - 2 mRNA levels were analyzed by RT - PCR.

In addition to managing the cell bank, the facility makes culture media and maintains a resale storeroom for laboratory products.

Shukla and colleagues discovered that a small drug molecule called BX795, which is sold to labs for use in experiments, helped clear HSV - 1 infection in cultured human corneal cells, in donated human corneas, and in the corneas of mice infected with HSV - 1.

Patent protection can also be sought for a new way to culture cells (a «method») or for a novel electrophoresis apparatus (a «machine»).

Setton's lab exposed the hiPSCs to a variety of different growth factors and culture media to coax them into first developing markers for, and then fully forming into, notochord cells.

For instance, to make a rod with collagen fibers aligned along its length (like a tendon) they cultured chondrocyte cells in a dog bone - shaped mold with loops on either end.

This study identifies myomerger as a fundmentally required protein for muscle development using cell culture and laboratory mouse models.

Despite being trapped and baked within a polymer, cell staining shows that the cultured cells remained alive, says Fernandez, an obvious necessity if they are to be used for artificial tissues.

The team pumped around 50 million rat liver cells into each of five bare scaffolds, then incubated the organs in culture for two weeks.

The Wyss team believes the ability of the human gut - on - a-chip to culture the microbiome with human gut cells also holds promise for the field of precision medicine, where a patient's own cells and gut microbiota could one day be cultured inside a gut - on - a-chip for testing different therapies and identifying an individualized treatment strategy.

With our human gut - on - a-chip, we can not only culture the normal gut microbiome for extended times, but we can also analyze contributions of pathogens, immune cells, and vascular and lymphatic endothelium, as well as model specific diseases to understand complex pathophysiological responses of the intestinal tract.»

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

Now researchers at the University of Plymouth, working in partnership with pharmaceutical company AstraZeneca, have for the first time successfully cultured and maintained cells from the guts of rainbow trout, a recommended fish species for toxicological studies.

«This research enables us to get one step closer to the optimal culture conditions for muscle stem cells.»

«If we win, the entire regulatory structure for autologous cell processing, with or without culture, will be rewritten such that any physician using good practices and treating patients responsibly can use stem cells as part of his or her medical practice,» he says.

New developments include reaction systems for catalysis research, sampling from reactors, systems for high - throughput applications in synthesis, the handling of microorganisms or cell - culturing procedures, and more.

To begin the research, the scientists screened human DIPG tumor cultures for surface molecules that could act as targets for CAR - T cells.

Also, researchers could carefully select which blood types to culture with each batch of stem cells, creating stockpiles of needed blood products for people with extremely rare blood types whose blood cell makeup makes it challenging to find good blood matches for transfusions because they would reject most other types of blood.

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

Milk glands, moreover, do not need the constant coddling required for cell cultures.

One of the 12 audience members was Ben Chao, a chemical engineer with a Ph.D. — not at all qualified to make a judgment on Scott's abilities in the area of «Approaches to Developing Serum - Free Media Formulations for Mammalian Cells in Culture.»

Although such harvested cells could be cultured as say, liver cells for treating hepatitis or dopamine - producing cells for Parkinson's, the resulting transplants would likely be rejected by patients» immune systems.

For example, cultured cells exhibit rhythmic oscillations in gene expression where there is no input from the master clock.

As it can take weeks to grow human cells into intact differentiated and functional tissues within Organ Chips, such as those that mimic the lung and intestine, and researchers seek to understand how drugs, toxins or other perturbations alter tissue structure and function, the team at the Wyss Institute for Biologically Inspired Engineering led by Donald Ingber has been searching for ways to non-invasively monitor the health and maturity of cells cultured within these microfluidic devices over extended times.

Although the bulk of the commercial manufacturing uses cultures of bacteria, such as Escherichia coli or Chinese hamster ovary cells, a few biotech companies are trying to produce therapeutic proteins in the milk of transgenic mammals (such as GTC Biotherapeutics, which is using goats; PPL Therapeutics, which is using sheep; and BioProtein Technologies, which is working with rabbits), transgenic chicken eggs (such as Avigenics or Vivalis), or even in transgenic crops (such as ProdiGene or Meristem Therapeutics); but it is early days for these «pharming» methods.

Oncologists William Hahn, Robert Weinberg, and colleagues at the Whitehead Institute for Biomedical Research in Cambridge, Massachusetts, mutated the gene for one part of the enzyme and inserted it into cultured human cells from colon, ovary, and breast tumors.

Sicor, for example, has recently acquired a plant in Vilnius, Lithuania, and Genentech a cell culture manufacturing facility in Porriño, Spain.

If it proves positive, the researchers will mix another portion of the sample with cultured bat and monkey cells to test for active virus.

The students used a 3 - D printer to design a smaller and more cost - efficient bioreactor — a miniaturized culture device where neural stems cells are grown and eventually become small laboratory brains for research.

The enzyme, called tankyrase, may prove useful for extending the lives of cultured cells grown to repair burned skin and other damaged tissue.

If a breast cancer line had silently contaminated Klonisch's cell culture early on, it would have been subject to the usual technique for immortalizing a normal cell (which involves applying enzymes, antibiotics and antibiotic - resistant genes).

Until recently, Ain was renowned for a highly prized repository of 18 immortal cancer cell lines, which he developed by harvesting tissue from his patients» tumors after removal, carefully culturing them to everlasting life in vials.

In addition, cell culture studies lasted two days in Gentzsch's study, whereas the clinical studies lasted for months.

Researchers have videotaped cultures in which embryos develop but found no visual pattern that hints at which cells are about to sprout, and staining for certain patterns of gene expression has been inconclusive.

Existing gels for cell culture are often animal derived, which can affect the reproducibility of results or, if synthetic, require coatings or coupling of cell - adhesive ligands.

In 2009, from experiments at the RIKEN Center for Developmental Biology in Kobe, Japan, he found that when culture conditions are right, adding a single ingredient — bone morphogenetic protein 4 (Bmp4)-- with precise timing is enough to convert embryonic cells to PGCs.

Using a labor - intensive cell culturing technique for hunting retroviruses that he had pioneered in the 1970s, Ruscetti had transmitted the pathogen from patients» T - cells to uninfected T - cells in the laboratory.

In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagen.

They are also trying to culture the undifferentiated cells for longer before adding Sonic hedgehog, so that they can grow larger numbers of cells.

«The concentrations of anabasine tested here are 100-fold lower than those required to inhibit growth of parasite cell cultures,» Palmer - Young says, «and we did not find any evidence for variation in anabasine sensitivity across parasite strains.

The researchers isolated the milk ducts and purified the breast - tissue cells to create a cell culture, which was then tested for different gene regulation profiles.

Previously, research groups had to get approval for each project involving human ES cells from their own Institutional Review Board (IRB) and then from a national committee under the Ministry of Education, Culture, Science, Sports, and Technology.

You can look for efficacy in cell cultures, but that tells you almost nothing about what's going to happen when you test in a mouse or a human.

«This pilot study showed that the culture system not only allowed for efficient propagation of sperm stem cells, but also eliminated ALL cells,» said Sadri - Ardekani.