Sentences with phrase «cut at the target site»
When inside the zygotes, the gRNA will seek out its target among the 3 X 109 nt of genetic content in the mouse genome and the Cas9 enzyme will make a cut at the target site.
http://blog.addgene.org/ Not exact matches
Scientists can guide the scissors to the place they want to cut in an organism's genetic instruction book with a guide RNA that matches DNA at the target site.
His team found that about 14 percent of the time cut - and - paste CRISPR / Cas9 made changes at nine of 12 possible «off - target» sites.
In both cases, the idea is to target an enzyme that cuts both strands of the double helix at a specific site.
They discovered that the DNA repair outcomes, namely the collection of insertions and deletions that are produced by the cellular repair machinery following DNA cutting, generate reproducible patterns at each target site.
Once injected inside a cell CRISPR / Cas9 will bind to the band - tailed pigeon genome at these target sites and cut the DNA.
Once injected inside a cell CRISPR / Cas9 will bind to the band - tailed pigeon genome at these target sites and cut the DNA.
«The proposal by German energy company E.ON to build a new coal - fired power station on this site which will emit up to 10m tonnes of C02 a year for the next 50 years are totally at odds with government targets for cutting CO2 emissions,» said Oakley.»