Sentences with word «cyclophilin»

Jeremy Luban and his colleagues at Columbia University in New York, and Heinrich Göttlinger's team at Harvard Medical School in Boston, studied the interaction of HIV with cyclophilin A in laboratory cultures.
The resulting cDNA (double line) has two different fates, which are determined by the sequence of the capsid and its interaction with Cyclophilin A proteins (red drops).
If cyclophilin A could be somehow switched off, HIV could be controlled in the body.
And it also tells scientists more about the family of proteins that includes cyclophilin A.
The data are summarized in Figure 2a — c. Values for the housekeeping gene cyclophilin A were highly consistent across all cells analyzed.
Expression of four stem cell marker genes normalized to cyclophilin in nine single cell equivalents prepared from a pool of nine lysed HES3 cells.
Luban and Göttlinger have both shown that disrupting the interaction between cyclophilin A and gag stops HIV - infected cells in culture from making new virus.
That raised an intriguing question, because one of the few things scientists already knew about cyclophilin A was that it is the target of a powerful immunosuppressant drug, cyclosporin A.
So far, our structures span protein families such as cyclophilins, kinases and proteases as well as pathways for biosynthesis, salvage and metabolism of isoprenoids, fatty acids, phospholipids, pyrimidines, purines and polyamines.
A cell was considered to be positive for a given marker if the value for ΔCt value was 21 cycles or less compared to cyclophilin levels within the same cell (individual data points, Figure 2a).
Data are expressed as delta Ct (ΔCt) marker vs. cyclophilin.
Cells negative for all markers studied (expressing cyclophilin only) were found primarily in the LOW and NEG populations (Figure 3c).
CT values for cyclophilin were somewhat more variable in cells isolated by flow cytometry and frozen prior to analysis, compared to freshly isolated and unmanipulated cells, but the gradients in patterns of gene expression were similar for cells isolated by the two techniques (Figure 3a — d).
Two teams have now shown that if cyclophilin A is inactivated, the virus can not reproduce itself.
A family of cis — trans peptidylprolyl isomerases that includes cyclophilins and FK506 - binding proteins (FKBPs).
Towards the center of the colony, ∼ 40 % of the cells had lost expression of all pluripotency markers, and we found a number of cells that were null - expressing no markers of pluripotency or lineage commitment, only the housekeeping gene cyclophilin a. However, many cells in this region still expressed Oct - 4.
The drug forms a complex with cyclophilin A and a third protein, calcineurin, which is needed to activate the T cells of the immune system.
Luban's group had shown earlier that cyclophilin A binds to HIV's most important structural protein, known as gag.
Other primate immunodeficiency viruses, such as SIV and HIV - 2, the rarer and less virulent human virus, do not need their host's cyclophilin A to replicate.
The protein, cyclophilin A, is a common constituent in cells, from bacteria to mammals.
But cyclophilin A is needed for so many other cellular activities that any treatment that inactivated it would probably be toxic.
What is clear is that the cyclophilin A is needed for replication.
Data are normalized to cyclophilin and expressed as ΔCt (marker gene — cyclophilin).
Crystal structure of TM1367 from Thermotoga maritima at 1.90 A resolution reveals an atypical member of the cyclophilin (peptidylprolyl isomerase) fold.
For each sample, expression of marker genes was normalized to cyclophilin.
Designed assays to evaluate novel drugs, with an emphasis on cellular pathways involving the enzymes poly - ADP - ribose polymerase, poly - ADP ribose glycohydrolase, cyclophilins and sirtuins.
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