Sentences with phrase «cyclophilin in»
Expression of four stem cell marker genes normalized to cyclophilin in nine single cell equivalents prepared from a pool of nine lysed HES3 cells.
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If cyclophilin A could be somehow switched off, HIV could be controlled in the body.
Jeremy Luban and his colleagues at Columbia University in New York, and Heinrich Göttlinger's team at Harvard Medical School in Boston, studied the interaction of HIV with cyclophilin A in laboratory cultures.
For expression analysis in mice, we used microarray data as described above to select two internal control genes, cyclophilin B (Cphn2) and ribosomal protein S3 (Rps3).
Q - PCR showed that ULK1 mRNA levels were increased 3 -7-fold in DAXX K / D samples, using the cyclophilin (CPH) internal control gene for normalization (Figure 6C).
Towards the center of the colony, ∼ 40 % of the cells had lost expression of all pluripotency markers, and we found a number of cells that were null - expressing no markers of pluripotency or lineage commitment, only the housekeeping gene cyclophilin a. However, many cells in this region still expressed Oct - 4.
The data are summarized in Figure 2a — c. Values for the housekeeping gene cyclophilin A were highly consistent across all cells analyzed.
Cells negative for all markers studied (expressing cyclophilin only) were found primarily in the LOW and NEG populations (Figure 3c).
CT values for cyclophilin were somewhat more variable in cells isolated by flow cytometry and frozen prior to analysis, compared to freshly isolated and unmanipulated cells, but the gradients in patterns of gene expression were similar for cells isolated by the two techniques (Figure 3a — d).