To obtain the pcDNA - SRα / FLAG vector, pcDNA3 - FLAG vector (Invitrogen Corp., Carlsbad, CA) was treated with HindIII and NruI to eliminate
the cytomegalovirus promoter fragment, blunted, and then ligated with 0.8 kb of ClaI - treated and Klenow filled - in SRα promoter fragment from pSRα vector (28).
In the current work we used adenoviral - type 5 (dE1 / E3)(
Cytomegalovirus promoter) with human ABCA10 transgene (Ad - h - ABCA10) purchased from Vector Labs ® in order to investigate whether gene therapy can be used as a pre-treatment to enhance the efficiency of inhaled cisplatin.
Not exact matches
Dwarki and Jaime Escobedo improved the AAV's ability to insert genes into chromosomes by adding a gene
promoter region from
cytomegalovirus, known to be active in the target for their gene therapy, muscle cells.