Sentences with word «cytometer»
A cytometer is a scientific instrument that is used to count and measure the characteristics of cells. It helps scientists to analyze and study cells more effectively. Full definition
The facility has 4 high throughput flow cytometer analysers with plate - reading capacity.
sanger.ac.uk
Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments Tricot S., Meyrand M., Sammicheli C., Elhmouzi - Younes J., Corneau A., Bertholet S., Malissen M., Le Grand R., Nuti S., Luche H., Cosma A Cytometry A., 2015 87 (4): 357 - 68.
The Countstar Fluorescence Cell Analyzer can do all that a traditional flow cytometer does but faster, easier, and at much lower cost.
The facility has 5 high speed flow cytometer sorters.
Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments
The facility supports a range of flow cytometric techniques and currently has 9 cytometers located at F2 - 33, F2 - 34, F2 - 35 and F2 - 36.
CytoFLEX LX is a new 6 - lasers 21 - parameter cytometer equipped with Avalanche photodiodes as detectors.
By purchasing the mass cytometer CYTOF, PHENOMIN - CIPHE became one of the first European laboratory to provide this service.
The purpose of the flow cytometry core facility is to sort cells for users, and to train facility users on the self - service cytometers and related software for data acquisition and analysis.
To use the analytical cytometers or the automated magnetic cell separator you must first be trained by FMCF staff.
He adds that «they can do all the things classical flow cytometers do, as well as look at images of individual cells.»
Our facility houses two third - generation Helios mass cytometers that can routinely analyze more than 40 markers per tube to provide an unprecedented «systems - level» view of phenotypic and functional variations among single cells within heterogeneous cell populations.
MilliporeSigma sells a range of options with its imaging flow cytometers, but whether an individual laboratory is buying a low - end unit or a core facility is installing a more complex one, researchers should expect to spend some time learning how to use these instruments.
Using a flow cytometer, Hawkins and his colleagues separated the green cells out from the mix, then grew them in a matrix.
I go on lab tours and find myself staring at rows upon rows of benches, glassware, PCR machines, flow cytometers, fluorescence microscopes, and more.
We had to figure out how to adapt the process for the flow cytometer.
Cell staining was evaluated with a Beckman Epics Elite flow cytometer (Beckman Coulter, Miami, FL) equipped with an air - cooled argon ion laser.
Cells treated with hamster anti-αβTCR or hamster murine γδTCR were washed three times in HBSS and incubated in PE - labeled Armenian hamster antihamster IgG (PharMingen) secondary antibody for 20 min at 4 °C, washed three additional times in PBS, fixed in 1 % paraformaldehyde, and assessed for fluorescence in a FACScan flow cytometer (Becton Dickinson).
Approach: Very little specialized equipment was needed for this study — apart from access to a flow cytometer to isolate single cells.
Sample acquisition was performed with a BD FACS Canto II flow cytometer and subsequent analysis was done using FlowJo software.
All flow cytometers are booked through the online booking calendar PPMS.
Peter O'Toole heads the Imaging and Cytometry Labs within the Technology Facility at the University of York which includes an array of confocal microscopes, flow cytometers and electron microscopes.
I am part of the flow cytometry core facility team and my primary responsibilities are to run the flow cytometer sorters, maintain the flow cytometer analysers and to provide technical and scientific expertise to our users.
Cells were fixed and acquisitions were performed using a BD LSR II flow cytometer (BD) and data were analyzed with FlowJo software (Tree Star, Ashland, OR).
The H - 2Dbgp33 — 41 MHC - peptide tetramer was used for Ag - specific CD8 T cell identification and was provided as fluorophore - conjugated tetramers by E.J.W.. All samples were acquired on a MACSQuant flow cytometer (Miltenyi Biotec) and analyzed using FlowJo software version 10.
Subsequently, aliquots of 100 μL (1 × 105 cells / mL) were incubated with 5 μL Annexin V - FITC and 5 μL propidium iodide for 15 minutes in the dark at room temperature and 1 × 104 ungated cells were counted using a flow cytometer (FACSCalibur, Becton Dickinson).
Cells were analyzed on a Stratedigm S1200Ex flow cytometer (Stratedigm, San Jose, CA) and data were analyzed using FlowJo v. 9.6 (Tree Star; Ashland, OR).
Mass cytometers analyze cells labelled with antibodies (or other markers) conjugated to metals, rather than fluorochromes, in a mass spectroscopy - coupled flow cytometer, also known as a CyTOF (latest generation units are known as Helios).
Guidelines for sample preparation will be discussed prior to preparation, and well before presentation of the samples to the cytometers.
Your financial support will go toward funding a study of the natural progression of West Nile virus in an effort to see how it moves through the human immune system, to help BSRI purchase new and much - needed equipment such as a flow cytometer, used to sort and analyze cells, or to search for undiscovered transmissible diseases that could infect the nation's blood supply.
Analysis of fluorescence took place in a FACSCalibur flow cytometer (BD Biosciences).
Bound immunoglobulin on target cells was labeled with FITC - conjugated donkey anti-human IgG (H+L)(Jackson ImmunoResearch) and detected using a Guava EasyCyte flow cytometer.
After 4 h, the number of migrated cells was quantified using a Guava EasyCyte flow cytometer.
We therefore excluded from our analysis the many genes whose expression is dependent upon the position of the cell within the cell cycle (e.g., S or M phase specific genes) by using a flow cytometer to collect only those with a 2N (G1) DNA content.
Tags for this Online Resume: microbiologist, flow cytometer, lab tech, ISO 9000, research tech, histologist
* Lab Instruments: Oscilloscope, Function generator, digital multimeter, Basic circuit elements, spectrophotometer, microscope, flow cytometer, hemocytometer, ventilators.
Job Description Summary Conducts production testing of assembled flow cytometers and related medical device products.