Sentences with phrase «cytometer cytof»

* Lab Instruments: Oscilloscope, Function generator, digital multimeter, Basic circuit elements, spectrophotometer, microscope, flow cytometer, hemocytometer, ventilators.

Tags for this Online Resume: microbiologist, flow cytometer, lab tech, ISO 9000, research tech, histologist

Bound immunoglobulin on target cells was labeled with FITC - conjugated donkey anti-human IgG (H+L)(Jackson ImmunoResearch) and detected using a Guava EasyCyte flow cytometer.

Analysis of fluorescence took place in a FACSCalibur flow cytometer (BD Biosciences).

Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments

The homogenized fresh breast tumor samples have been represented for flow cytometric analysis using FACS calibur flow cytometer (Becton Dickinson, Sunnyvale, CA, USA) equipped with a compact air cooked low power 15 mwat argon ion laser beam (488nm), data analysis was conducted using DNA analysis program MODFIT (Topsham, USA).

CytoFLEX LX is a new 6 - lasers 21 - parameter cytometer equipped with Avalanche photodiodes as detectors.

The H - 2Dbgp33 — 41 MHC - peptide tetramer was used for Ag - specific CD8 T cell identification and was provided as fluorophore - conjugated tetramers by E.J.W.. All samples were acquired on a MACSQuant flow cytometer (Miltenyi Biotec) and analyzed using FlowJo software version 10.

Prof. Dr. Mona Samy, at Flow cytometry Unit - Mansoura Children Hospital, has given help and valuable information regarding application of flow cytometer analysis.

The facility has 4 high throughput flow cytometer analysers with plate - reading capacity.

All flow cytometry data were acquired on an LSRII cytometer (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (Tree Star, Ashland, OR).

Approach: Very little specialized equipment was needed for this study — apart from access to a flow cytometer to isolate single cells.

We propose cytometer analysis using up to 20 different parameters, Fluorescent microscopy, in vitro restimulation cultures, qRT - PCR analysis, ELISA and ELISPOT.

Cells treated with hamster anti-αβTCR or hamster murine γδTCR were washed three times in HBSS and incubated in PE - labeled Armenian hamster antihamster IgG (PharMingen) secondary antibody for 20 min at 4 °C, washed three additional times in PBS, fixed in 1 % paraformaldehyde, and assessed for fluorescence in a FACScan flow cytometer (Becton Dickinson).

Cell staining was evaluated with a Beckman Epics Elite flow cytometer (Beckman Coulter, Miami, FL) equipped with an air - cooled argon ion laser.

We had to figure out how to adapt the process for the flow cytometer.

Using a flow cytometer, Hawkins and his colleagues separated the green cells out from the mix, then grew them in a matrix.

The Countstar Fluorescence Cell Analyzer can do all that a traditional flow cytometer does but faster, easier, and at much lower cost.

He adds that «they can do all the things classical flow cytometers do, as well as look at images of individual cells.»

I go on lab tours and find myself staring at rows upon rows of benches, glassware, PCR machines, flow cytometers, fluorescence microscopes, and more.

All flow cytometers are booked through the online booking calendar PPMS.

Peter O'Toole heads the Imaging and Cytometry Labs within the Technology Facility at the University of York which includes an array of confocal microscopes, flow cytometers and electron microscopes.

The facility supports a range of flow cytometric techniques and currently has 9 cytometers located at F2 - 33, F2 - 34, F2 - 35 and F2 - 36.

Mass cytometers analyze cells labelled with antibodies (or other markers) conjugated to metals, rather than fluorochromes, in a mass spectroscopy - coupled flow cytometer, also known as a CyTOF (latest generation units are known as Helios).

Guidelines for sample preparation will be discussed prior to preparation, and well before presentation of the samples to the cytometers.

Our facility houses two third - generation Helios mass cytometers that can routinely analyze more than 40 markers per tube to provide an unprecedented «systems - level» view of phenotypic and functional variations among single cells within heterogeneous cell populations.

The purpose of the flow cytometry core facility is to sort cells for users, and to train facility users on the self - service cytometers and related software for data acquisition and analysis.

To use the analytical cytometers or the automated magnetic cell separator you must first be trained by FMCF staff.

Job Description Summary Conducts production testing of assembled flow cytometers and related medical device products.