Sentences with phrase «cytometric analysis of»

(A, B) Mixed (1:1) CD11cDTR (CD45.1 +) and Ebi2 - / - or Ebi2 + / + (CD45.1 ʱCD45.2 +) BM chimeric mice were immunized with SRBCs and flow cytometric analysis of B cell responses performed five days later.

Flow cytometric analysis of BT474, MCF7 / HER2, or MCF7 target cells after incubation with supernatant from HB1.F3.H2IgG, HB1.F3.Adeno - H2IgG, or HB1.F3.Lenti - H2IgG (C), followed by incubation with FITC - conjugated anti-human IgG (blue histograms).

Flow cytometric analysis of asialoglycoprotein receptor (ASGPR) levels on the surface of hepatocytes, which were obtained from the liver specimens of patients that received hepatectomy, were used as predictors of liver dysfunction after majo...

Flow cytometric analysis of rabbit anti-CD3 (SP7) antibody ab16669 (1/100) in Jurkats cells (green) compare to negative control of rabbit IgG (blue).

Flow cytometric analysis of the cells following anti-CD8 antibody and complement treatment indicated that < 2 % were CD8 +.

(B, C) Flow cytometric analysis of HIV - 1 gene expression in (B) mock infected or (C) latently infected CD4 + T cells under non-polarizing conditions, either at the basal state or after reactivation with antibodies against CD3 and CD28.

Not exact matches

Although conventional flow cytometry is limited to measuring particles down to approximately 300 nm — 500 nm, a relatively new flow - cytometric method — called «imaging flow cytometry» — allows for the analysis of EVs smaller than 300 nm.

Flow cytometric analysis revealed that anti-Thy 1.2 antibody plus complement depleted > 97 % of the Thy 1.2 + cells (data not shown).

The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design.

Additionally, an aliquot of 4 × 106 splenocytes was stained for Foxp3 expression using methods and reagents, provided by the manufacturer (eBioscience), prior to flow cytometric analyses.

Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.

Assisted / Supervised use The facility's staff will assist researchers in using FRACTAL strumentation, and will provide information for the preparation of samples for cytometric analysis.

Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS analysis.