Sentences with word «cytometry»

Flow cytometry analysis using mouse blood showed that MSCs were released from bone marrow to the blood in cinnamtannin B -1-administered mice.
This reprogramming to an ESC like state was also accompanied by an increase in proliferation as shown by flow cytometry analysis (FACS).
Spleen cell suspensions that were enriched or depleted of CD8 + cells were examined by flow cytometry for the relative percent of αβTCR +, γδTCR +, and Thy 1 + cells.
Quantitative Flow Cytometry in Cell Therapy Ines Mende, PhD, Hitachi Chemical Advanced Therapeutic Solutions, LLC
Dr. Ruxandra Sîrbulescu analyzes flow cytometry data on immune cells used in a wound healing study.
Follow the instructions above to register your laboratory with the Flow and Mass Cytometry Core facility
GFP + leukemia cells in peripheral blood were counted by flow cytometry on day 7 (C) and day 14 (D).
A Fab fragment was produced from the whole molecule mAb 5.91 and used in flow cytometry assays as a model for the recombinant scFv version of the antibody by May, 2011.
Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein - tagged cells, assessing apoptosis and cell cycle status, the identification / isolation of stem and cancer stem cells (e.g., marker positive, side - population, etc.) and the deposition of single cells.
PHENOMIN - CIPHE, on the strength of its expertise in genetics and mouse immunology, is positioned as a pioneer in multiparametric analysis of the immune system of mutant mice in normal conditions or exposed to risks of infection, by an integration of innovative research and technical knowledge in the field of flow and mass cytometry as well as advanced microscopy.
Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments Tricot S., Meyrand M., Sammicheli C., Elhmouzi - Younes J., Corneau A., Bertholet S., Malissen M., Le Grand R., Nuti S., Luche H., Cosma A Cytometry A., 2015 87 (4): 357 - 68.
To determine whether the growth advantage conferred by X4 - ZFNs treatment in the presence of X4 - and R5X4 - HIV resulted from a survival advantage of CXCR4 disrupted cells, we performed flow cytometry at various time points post infection as well as deep sequencing of the X4 - ZFNs target site on HIV - infected and uninfected cultures.
* Note: The new booking system only impacts mass cytometry services.
EL helped to carry out the optimization of hypoxia / reoxygenation protocol, carried out the flow cytometry experiments and animal experiments, and prepared the manuscript.
The facility also focuses on developing the mass - cytometry technology, new experimental assays and reagents as well as novel data analysis tools.
OMIP - 016: Characterization of antigen - responsive macaque and human T — cells Guenounou S., Bosquet N., Dembek C.J., Le Grand R., Cosma A. Cytometry PART A, 2013 83 (2): 182 - 4.
«Flow cytometry identified a large population of apoptotic melanoma cells, and single - cell electrophoresis indicated that mensacarcin causes genetic instability, a hallmark of early apoptosis,» Loesgen said.
Third, it is significantly less expensive to purchase and run than any of the traditional flow cytometry systems.
CXCR4 cell surface and intracellular expression was detected by flow cytometry after co-staining with the N - terminal specific CXCR4 antibody 4G10 and the extracellular loop (ECL) specific antibody 12G5 whose epitope includes the CXCR4Δ18 deleted residues [42].
The facility is continuously expanding with the newest software and machinery for both sort and analysis capabilities of up to 18 fluorescent parameters, and is committed to meet all of the flow cytometry needs for the Hubrecht Institute, the UMC Utrecht and the Princess Máxima Center.
A. Mietke, O. Oliver, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aland, J. Guck, E. Fischer - Friedrich Extracting cell stiffness from Real - Time Deformability Cytometry — a theoretical and experimental analysis.
The Immune Monitoring and Flow Cytometry Resource provides multiple services including isolation and cryopreservation of PBMCs and plasma / serum for clinical trials, as well as a spectrum of immunoassays and can develop customized immunoassays.
Identify immune cell populations with our flow cytometry antibodies and kits and isolate them with our cell selection kits.
VIM offers the following services for pig / ruminant samples: ELISA, FACS, in vitro restimulation cultures, qRT - PCR analysis, fluorescence laser microscopy, 3 - 4 laser cytometry (405, 488, 560 and 640 nm) allowing the simultaneous monitoring of up to 20 parameters.
It creates customisable and high - scale mouse models of human disease or displaying an abnormality (KI, KO mouse models) and it provides a complete phenotyping characterisation of the immune system by polychromatic and mass cytometry measuring more than 50 simultaneous quantitative parameters in standardised conditions.
With its image cytometry capabilities, you can quickly and easily generate per - cell data, whether you're using fixed or live cells, performing end - point assays, or taking kinetic measurements over time — opening up a broad range of cell - based applications.
An alternative counting method called flow cytometry requires more expensive equipment and greater expertise to get reliable data.
Using an eGFP - positive biological calibrator to optimize imaging flow cytometry acquisition and analysis parameters
He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.
A comparison of the papers made byNature reporters has revealed two pairs of flow cytometry plots that seem to be duplicates.
All of the following antibodies for both analytical and cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.
Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.
Capacitance cytometry: Measuring biological cells one by one.
He has ongoing collaborations with many leading microscopy and cytometry companies and his group also provides research support to many academics and commercial organisations.
For details on how to make booking, please visit Cytometry confluence page.
Peter O'Toole heads the Imaging and Cytometry Labs within the Technology Facility at the University of York which includes an array of confocal microscopes, flow cytometers and electron microscopes.
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