Sentences with phrase «cytometry analysis»

ICS analysis, mass cytometry analysis, in vivo imaging, methodology and statistical consultancy, flow and mass cytometry courses, in vivo imaging courses.

Cell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analysis.

(A and B) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen on indicated days for syngeneic and allogeneic mice.

(C and D) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen of control mice and VPA recipients on the indicated days.

Using two different viability exclusion dyes via flow cytometry analysis, we observed no difference in the viability of ILCs treated with or without lethal toxin and / or IL - 23 (Fig 1D, 1E and 1F and S1A and S1B Fig).

Flow cytometry analysis showed that, compared with control, VPA treatment markedly reduced the frequencies of Th1 and Th17 cells in the spleen (Fig. 2C, 2D) and MLN (Supplemental Fig. 3A, 3B).

Three - color flow cytometry analysis was performed to detect the CD44 + / CD24 - / ESA + cell population.

(b) The same flow cytometry analysis for BCM2 (total cell population) and BCM2 F3 (cycling hypoxia - selected subpopulation) cells.

Flow cytometry analysis of the total population was performed seven days later to determine the CD81 KO efficiency and overall pluripotency levels.

Analytical flow cytometry analysis was performed on whole blood after lysis of erythrocytes and fixing in 2 % PFA.

Flow cytometry analysis using mouse blood showed that MSCs were released from bone marrow to the blood in cinnamtannin B -1-administered mice.

Not exact matches

The centre will be structured around six technical platforms focusing on zebrafish transgenesis and micromanipulation, bioimaging, mouse transgenesis, flow cytometry, stem cell culture, and gene - expression analysis.

The researchers then used an array of analytical tools — including stool and urine analysis, flow cytometry, light microscopy, nuclear magnetic resonance spectroscopy, and 16S rRNA analysis — to observe the wide - ranging effects of this sequential co-infection.

Although conventional flow cytometry is limited to measuring particles down to approximately 300 nm — 500 nm, a relatively new flow - cytometric method — called «imaging flow cytometry» — allows for the analysis of EVs smaller than 300 nm.

Using an eGFP - positive biological calibrator to optimize imaging flow cytometry acquisition and analysis parameters

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

This webinar will introduce viewers to the challenges, limitations, and pitfalls of flow cytometry - based EV analysis, and to the imaging flow cytometry methodology.

The facility is continuously expanding with the newest software and machinery for both sort and analysis capabilities of up to 18 fluorescent parameters, and is committed to meet all of the flow cytometry needs for the Hubrecht Institute, the UMC Utrecht and the Princess Máxima Center.

The department has expertise and infrastructure to support bacterial and viral analysis, and the detailed evaluation of cellular and serological immune responses including flow cytometry and cell sorting, Luminex multiplex platforms, real time PCR, microarrays, comprehensive histological staining, immunohistochemistry and in - situ hybridisation.

The flow cytometry facility at the Wellcome Sanger Institute is a dedicated scientific service offering investigators with state of art flow cytometry instrumentation together with assistance in running samples, data analysis and experimental design.

The Institute is currently running on a Site License by Flowjo, a flow cytometry data analysis software package which offers a range of analysis platforms that includes compensation, cell cycle, proliferation studies and kinetics.

Prof. Dr. Mona Samy, at Flow cytometry Unit - Mansoura Children Hospital, has given help and valuable information regarding application of flow cytometer analysis.

A. Mietke, O. Oliver, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aland, J. Guck, E. Fischer - Friedrich Extracting cell stiffness from Real - Time Deformability Cytometry — a theoretical and experimental analysis.

The current study involved monitoring the patients for one year after completed stem cell transplantation and used mass cytometry to study the different cell types of the immune system and the so called ProSeek method for simultaneous protein analysis.

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

Flow cytometry and high - content analysis experimental data will be presented to demonstrate performance in receptor occupancy, antibody internalization, and effector function assays using antibodies against CD20, CD71, and HER2 in breast cancer and leukemia models.

Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.

Mass - cytometry provides state - of - the art single cell proteomics, including extensive data analysis as a support for this novel and complicated technology.

Flow cytometry Resource, Advanced Cytometry Technical Applications Laboratory (FRACTAL) is a core facility of IRCCS Ospedale San Raffaele that offers state - of - the - art instrumentation and analysis techniques to the scientific ccytometry Resource, Advanced Cytometry Technical Applications Laboratory (FRACTAL) is a core facility of IRCCS Ospedale San Raffaele that offers state - of - the - art instrumentation and analysis techniques to the scientific cCytometry Technical Applications Laboratory (FRACTAL) is a core facility of IRCCS Ospedale San Raffaele that offers state - of - the - art instrumentation and analysis techniques to the scientific community.

For these reasons, mass cytometry has emerged as the leading technology for high - content single - cell analysis in health and disease.

The eBioscience product line contains a comprehensive and innovative portfolio of fluorochrome conjugated antibodies and reagents for immunology, oncology, and multicolor flow cytometry, as well as highly - validated ELISA and multiplex immunoassay kits, recombinant proteins, and general - purpose lab reagents for complete biological system analysis.

We make use of mass - cytometry to perform multi-dimensional analysis of up to 40 - 45 parameters in millions of single cells.

PHENOMIN - CIPHE, on the strength of its expertise in genetics and mouse immunology, is positioned as a pioneer in multiparametric analysis of the immune system of mutant mice in normal conditions or exposed to risks of infection, by an integration of innovative research and technical knowledge in the field of flow and mass cytometry as well as advanced microscopy.

We acknowledge the OMRF FACS core and the OUHSC Flow Cytometry and Imaging Core for cell sorting and analysis.

Analysis software for flow cytometry: Summit software; BD DiVa software; CXP; Gallios Software; Cell Quest; FCS Express

The purpose of the flow cytometry core facility is to sort cells for users, and to train facility users on the self - service cytometers and related software for data acquisition and analysis.

VIM offers the following services for pig / ruminant samples: ELISA, FACS, in vitro restimulation cultures, qRT - PCR analysis, fluorescence laser microscopy, 3 - 4 laser cytometry (405, 488, 560 and 640 nm) allowing the simultaneous monitoring of up to 20 parameters.

Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein - tagged cells, assessing apoptosis and cell cycle status, the identification / isolation of stem and cancer stem cells (e.g., marker positive, side - population, etc.) and the deposition of single cells.

Through the analysis of breast cancer patient blood, lymph node, and tumor samples by high - dimensional flow cytometry and in vitro functional assays we hope to unravel some of the answers to these questions.

CT values for cyclophilin were somewhat more variable in cells isolated by flow cytometry and frozen prior to analysis, compared to freshly isolated and unmanipulated cells, but the gradients in patterns of gene expression were similar for cells isolated by the two techniques (Figure 3a — d).

SSI offers vaccination studies in mice including 1 - 2 control groups and full immunogenicity analysis including CMI and humoral immunogenicity using Flow cytometry (B and T cells) and ELISA (cytokines and antibodies).

Prior to working at City of Hope, she worked as a research associate in Dr. Peter Lee's lab at Stanford University since 2006 and facilitated in investigating mechanisms causing immune dysfunction in melanoma and breast cancer patients» peripheral blood, lymph node and tumor specimens utilizing techniques including microarray analysis, RT - PCR, Phospho - flow cytometry, histoloogy, and functional FACS studies.

Often high tech testing like flow cytometry or DNA analysis are required to confirm a lymphoma diagnosis in a cat.

Familiar with low cytometry, immunohistochemistry, image analysis, electron microscopy, automation and molecular diagnostic procedures

Highlights Advanced knowledge of biological engineering Knowledge of flow cytometry Plasmid design Adept at batch inventory and database maintenance Knowledge of next generation sequencing Strong technical skills Efficient at multitasking and prioritizing tasks Highly analytical Superior work ethic Team collaborator Experience Biological Research Analyst 2/14/2016 — Current BioMax Industries — Holkley, NY Collect, evaluate and document data and experiment computations with scientific analysis software.

Microscopy Tissue Culture Multi-Color Flow Cytometry Assay Development High - Content Imaging Protein Expression & Purification Techniques Molecular Genetics Techniques Western Blot ELISA Immunofluorescence Microscopy Human Primary Cell Culture Data Analysis

Professional Summary: * Superior problem solving skills, QC / QA validation, GLP protocol expert, FDA facility and 510k * qPCR designs, Elisa, SOP validation, Automated System (Tecan), Statistical analysis * HPLC, Flow Cytometry, Chromotography, Analytical Chemistry, Real Time PCR

Tags for this Online Resume: High - Throughput (HTS) and High - Content Screening (HCS), Research Scientist, Laser Scanning Confocal Microscopy and Spectrophotometry Usage and Analysis, Murine manipulation, • ELISA • Western Blot, Flow Cytometry, Microbiology and Immunology, cell - based assays, molecular biology techniques

Professional Summary: * Superior problem solving skills, QC / QA validation, GLP protocol expert, FDA * qPCR designs, Elisa, SOP validation, Automated System Specialist, Statistical analysis * HPLC, Flow Cytometry, Chromotography, Analytical Chemistry, Sample preparation and analysis * HPLC, Flow Cytometry, Chromotography, Analytical Chemistry, Sample preparation and AnalysisAnalysis