Sentences with phrase «cytometry antibodies»

Identify immune cell populations with our flow cytometry antibodies and kits and isolate them with our cell selection kits.

Not exact matches

CST has about 225 employees, and specializes in making antibodies to be used in research applications such as immunohistochemistry and flow cytometry.

Secondary antibodies play an important role in many research applications, including immunofluorescence, immunohistochemistry, Western blot, ELISA, flow cytometry, and more.

They have screened combinations of antibodies that bind to specific proteins on the surface of the immature and mature stem cells and that can be used for flow cytometry, a common laboratory technique for sorting cells.

All of the following antibodies for both analytical and cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.

CXCR4 cell surface and intracellular expression was detected by flow cytometry after co-staining with the N - terminal specific CXCR4 antibody 4G10 and the extracellular loop (ECL) specific antibody 12G5 whose epitope includes the CXCR4Δ18 deleted residues [42].

The immune responses will be evaluated in the different species by measuring influenza antigen - specific IgG / IgA and IgM, neutralising antibodies and antibodies inhibiting hemagglutination and antigen specific lymphocyte responses and cytokine secretion profiles by advanced flow cytometry.

Moreover, the allospecific apoptotic activity of the putative DN T cells was abolished in two separate experiments using in vitro treatment with anti-Thy 1.2 antibody plus complement, which removed > 97 % of the Thy 1 + cells, as determined by flow cytometry.

Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes.

This antibody stains approximately 50 % of NK cells, 30 - 40 % of CD4 + and CD8 + T cells and 15 - 30 % B cells by flow cytometry.

HL - 60 cells were stained with 1 µg / mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Proteintech offers a variety of secondary antibodies suitable for Western Blotting, ELISA, cellular imaging, and flow cytometry.

Individual, edited (CD81 knockout) hiPS cells were expanded into clonal lines and analyzed for expression of CD81 and three pluripotency markers via flow cytometry using antibodies against CD81, Oct ‑ 4, TRA ‑ 1 ‑ 60, and SSEA - 4.

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

Flow cytometry and high - content analysis experimental data will be presented to demonstrate performance in receptor occupancy, antibody internalization, and effector function assays using antibodies against CD20, CD71, and HER2 in breast cancer and leukemia models.

Oligonucleotide - labeled antibodies allow integration of cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed protein markers far surpassing what can be measured by cytometry - based approaches.

The two nodes perform standardized mass - cytometry experiments on customer provided samples using validated panels of metal - coupled antibodies.

Complete phenotyping of the mouse immune system by polychromatic and mass cytometry (CYTOF), thanks to a set of standardised protocols enabling isolation of viable cells from lymphoid and non-lymphoid organs (lung, skin, intestine,...) for labelling using complex ranges of antibodies whose compatibility allows the simultaneous registration of 50 quantitative parameters at least (size, structure, specific antibodies and cell viability).

The eBioscience product line contains a comprehensive and innovative portfolio of fluorochrome conjugated antibodies and reagents for immunology, oncology, and multicolor flow cytometry, as well as highly - validated ELISA and multiplex immunoassay kits, recombinant proteins, and general - purpose lab reagents for complete biological system analysis.

The institute facilities grouped into the CurieCoreTech include advanced imaging, high throughput sequencing, bioinformatics, reverse phase protein array, proteomics and mass spectrometry, antibody technologies, cytometry, animal housing, and a unique experimental radiotherapy platform (https://science.institutcurie.org/platforms).

Boster now offers 1300 + rabbit monoclonal antibodies validated for Western Blotting (WB), ICC, IHC - P, IHC - F and Flow Cytometry / FACS.

A diverse portfolio of high - quality primary and secondary antibodies is available for use in cancer research, epigenetics, immunology, neuroscience, and stem cell research as well as validated for multiple applications, including flow cytometry, western blotting, and immunoprecipitation.

Cell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analysis.

Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using fluorescently labeled antibodies.

Differentiated mouse Th1 cells (A, B, C) and naïve mouse CD4 cells (D, E, F) were analyzed by flow cytometry for (A, D) T - bet expression using Human T - bet / TBX21 Alexa Fluor ® 488 - conjugated Antibody (Catalog # IC53851G), (B, E) TNF - alpha expression using Mouse TNF - alpha PE - conjugated Antibody (Catalog # IC410P), and (C, F) IL - 18R alpha expression using Mouse IL - 18 R alpha / IL - 1 R5 APC - conjugated Antibody (Catalog # FAB1216A).

We then harvested single cells and labeled them with antibodies GCTM - 2 and TG30 for flow cytometry.

SSI offers vaccination studies in mice including 1 - 2 control groups and full immunogenicity analysis including CMI and humoral immunogenicity using Flow cytometry (B and T cells) and ELISA (cytokines and antibodies).

For surface expression of CD36 and MARCO, alveolar macrophages were stained with CD36 - APC and MARCO - PE antibodies and surface expression was quantified by flow cytometry.

A Fab fragment was produced from the whole molecule mAb 5.91 and used in flow cytometry assays as a model for the recombinant scFv version of the antibody by May, 2011.

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