Sentences with phrase «cytometry assays»

A Fab fragment was produced from the whole molecule mAb 5.91 and used in flow cytometry assays as a model for the recombinant scFv version of the antibody by May, 2011.

While mass cytometry offers a powerful and highly multiplexed method for profiling single cells, there are various technical considerations for researchers wanting to perform a mass cytometry assay for the first time, or at a more advanced level.

Noto A, Ngauv P, Trautmann L. Cell - based Flow Cytometry Assay to Measure Cytotoxic Activity.

Not exact matches

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

IDMIT will contribute 1) To the development and validation of assays based on flow cytometry and mass cytometry for the evaluation of immune responses in humans and animal models; these tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal model platform, in particularly by providing access to NHP models and to new technologies for in vivo imaging infections and host responses; 3) To networking activities, in particular by organising a workshop on in vivo imaging.

Experts and Key Opinion Leaders on the topic will share their experiences and views on potency assays and cell characterization by flow cytometry.

[B and E] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for leukocyte surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

Flow cytometry and high - content analysis experimental data will be presented to demonstrate performance in receptor occupancy, antibody internalization, and effector function assays using antibodies against CD20, CD71, and HER2 in breast cancer and leukemia models.

Cell death induced by radiation as well as upon inhibition of these pathways was measured by TUNEL assay using flow cytometry.

[B] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

Oligonucleotide - labeled antibodies allow integration of cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed protein markers far surpassing what can be measured by cytometry - based approaches.

This novel assay allows the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell surface and intracellular proteins using standard flow cytometry.

MITO - ID ® Membrane Potential Detection Kit for Microscopy and Flow Cytometry The Only Assay that Monitors Energetic Status Using a Simple Mix - and - Read, No - Wash Protocol

This adaptation of Proximity Ligation Assay expands the capabilities of flow cytometry to include data relating to localization of proteins to and within organelles.

Through the analysis of breast cancer patient blood, lymph node, and tumor samples by high - dimensional flow cytometry and in vitro functional assays we hope to unravel some of the answers to these questions.

I mastered various blotting techniques; performed complex, cell - based assays; and become skilled in the use of flow cytometry (FACS) technology to complete advanced cell sorting, cell counting and biomarker detection.

Performed Inventory and Procurement oversight and assisted and trained all students in activities such as Plasmid Construction, Elutriation of Whole Blood, Dendritic Cell Studies, Organ / Tissue Processing, Ctl Assays, Cell Culture Maintenance, Transfection, Animal Handling, Flow Cytometry and Microscopy.

Microscopy Tissue Culture Multi-Color Flow Cytometry Assay Development High - Content Imaging Protein Expression & Purification Techniques Molecular Genetics Techniques Western Blot ELISA Immunofluorescence Microscopy Human Primary Cell Culture Data Analysis

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virologyassays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology VirologyAssays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS

Laboratory: Experience with laboratory applications in parasitology, immunology and molecular biology including, microscopy, BCA Protein Assay, ELISA, Flow Cytometry, Real Time qPCR, Luminex, Gas chromatography (GC) mass spectrometry, cotinine assays (saliva), magnetic bead cell separation (MACS, Percoll and Ficoll) and whole blood cultures.

Tags for this Online Resume: High - Throughput (HTS) and High - Content Screening (HCS), Research Scientist, Laser Scanning Confocal Microscopy and Spectrophotometry Usage and Analysis, Murine manipulation, • ELISA • Western Blot, Flow Cytometry, Microbiology and Immunology, cell - based assays, molecular biology techniques