Sentences with phrase «cytometry data»

Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

In accordance with the flow cytometry data, expression of AcGFP1 in gesicle - treated cells was knocked out in the majority of cells, despite AcGFP1 being expressed in nearly all control cells.

The Institute is currently running on a Site License by Flowjo, a flow cytometry data analysis software package which offers a range of analysis platforms that includes compensation, cell cycle, proliferation studies and kinetics.

All flow cytometry data were acquired on an LSRII cytometer (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (Tree Star, Ashland, OR).

Dr. Ruxandra Sîrbulescu analyzes flow cytometry data on immune cells used in a wound healing study.

The scientists used diffusion maps — a basic way to order snapshots of single cells along their developmental journey — but they plan to study developmental trajectories using a new algorithm called Wishbone, which uses single - cell RNA sequencing and mass cytometry data to specifically model branching steps during cell differentiation (Nat Biotechnol, 34:637 - 45, 2016).

The two plots in the Nature paper show flow cytometry data - a counting up of cells - after exposure to other cells in bone marrow taken from younger and older mice.

Not exact matches

Flow cytometry and sorting data were analyzed with FlowJo software (Treestar) or Cellquest (BD Biosciences), in the case of primary cells.

The flow cytometry facility at the Wellcome Sanger Institute is a dedicated scientific service offering investigators with state of art flow cytometry instrumentation together with assistance in running samples, data analysis and experimental design.

Someone who knows a thing or two about the importance of data collection is Michael Andreeff, M.D., Ph.D., professor of Leukemia, who was a pioneer in the develop of flow cytometry, a method for counting and sorting cells that is commonly used in today's clinical trials.

These data obtained from Flow cytometry Unit, Mansoura Children Hospital.

Flow cytometry and high - content analysis experimental data will be presented to demonstrate performance in receptor occupancy, antibody internalization, and effector function assays using antibodies against CD20, CD71, and HER2 in breast cancer and leukemia models.

Mass - cytometry provides state - of - the art single cell proteomics, including extensive data analysis as a support for this novel and complicated technology.

The purpose of the flow cytometry core facility is to sort cells for users, and to train facility users on the self - service cytometers and related software for data acquisition and analysis.

Multiplexed Imaging Here the goal is to add spatial resolution to cytometry - data in order to understand, in a tissue, WHERE immune and cancer cells interact We design, develop and apply methods for multiplexed visualization of protein and RNA molecules in tissue sections.

This adaptation of Proximity Ligation Assay expands the capabilities of flow cytometry to include data relating to localization of proteins to and within organelles.

Figure S1 shows QRT - PCR data for expression of the TGF - beta superfamily member GDF3, a gene which is strongly downregulated across the various subpopulations, for four ES cell lines subjected to separation by flow cytometry.

Highlights Advanced knowledge of biological engineering Knowledge of flow cytometry Plasmid design Adept at batch inventory and database maintenance Knowledge of next generation sequencing Strong technical skills Efficient at multitasking and prioritizing tasks Highly analytical Superior work ethic Team collaborator Experience Biological Research Analyst 2/14/2016 — Current BioMax Industries — Holkley, NY Collect, evaluate and document data and experiment computations with scientific analysis software.

Microscopy Tissue Culture Multi-Color Flow Cytometry Assay Development High - Content Imaging Protein Expression & Purification Techniques Molecular Genetics Techniques Western Blot ELISA Immunofluorescence Microscopy Human Primary Cell Culture Data Analysis