Sentences with phrase «cytometry for»

(E) Cyp7b1 + / − or Cyp7b1 − / − recipients were reconstituted with WT or lymphotoxin (LTβ10) transgenic (tg) BM cells and analyzed by flow cytometry for the indicated markers.

Schaub, F.X., et al. (2015) Fluorophore - NanoLuc BRET reporters enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis.

(2015) Fluorophore - NanoLuc BRET reporters enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis.

Differentiated mouse Th1 cells (A, B, C) and naïve mouse CD4 cells (D, E, F) were analyzed by flow cytometry for (A, D) T - bet expression using Human T - bet / TBX21 Alexa Fluor ® 488 - conjugated Antibody (Catalog # IC53851G), (B, E) TNF - alpha expression using Mouse TNF - alpha PE - conjugated Antibody (Catalog # IC410P), and (C, F) IL - 18R alpha expression using Mouse IL - 18 R alpha / IL - 1 R5 APC - conjugated Antibody (Catalog # FAB1216A).

In vitro expanded ILCs were stimulated with IL - 1β, IL - 23, PMA, ionomycin or a combination of these stimuli for 5 hr in presence of brefeldin A. Cells were analyzed by ICS and flow cytometry for IL - 22 and GM - CSF.

Spleen cell suspensions that were enriched or depleted of CD8 + cells were examined by flow cytometry for the relative percent of αβTCR +, γδTCR +, and Thy 1 + cells.

IDMIT will contribute 1) To the development and validation of assays based on flow cytometry and mass cytometry for the evaluation of immune responses in humans and animal models; these tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal model platform, in particularly by providing access to NHP models and to new technologies for in vivo imaging infections and host responses; 3) To networking activities, in particular by organising a workshop on in vivo imaging.

Not exact matches

A new joint International Dairy Federation (IDF) / International Organization for Standardization (ISO) Standard (ISO 19344 IDF 232) has just been released to provide a method for the quantification of lactic acid bacteria by flow cytometry in fermented products, starter cultures, and probiotics used in dairy products...

The kit features true multiplexing capabilities with green fluorescent protein (GFP) and other green fluorescent probes, and is optimized for both fluorescence microscopy and flow cytometry applications.

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

Also covered will be techniques for analyzing exosomes, microvesicles, and apoptotic bodies in unprocessed samples, how imaging flow cytometry can be used to evaluate or reevaluate EV isolation techniques, and the advantages and disadvantages of using this method.

Her current research focuses on developing clinical applications for imaging flow cytometry, with special attention to transcription factor pathways.

Although conventional flow cytometry is limited to measuring particles down to approximately 300 nm — 500 nm, a relatively new flow - cytometric method — called «imaging flow cytometry» — allows for the analysis of EVs smaller than 300 nm.

Imaging flow cytometry (IFC) has emerged as a useful and efficient tool for studying the signaling pathways in immunophenotypically defined subpopulations of immune cells.

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

They have screened combinations of antibodies that bind to specific proteins on the surface of the immature and mature stem cells and that can be used for flow cytometry, a common laboratory technique for sorting cells.

All of the following antibodies for both analytical and cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.

IDMIT is an infrastructure for preclinical research in infectious diseases and immunology which is certified ISO9001 and which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology, flow cytometry and mass cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).

Institutional needs for flow cytometry services were carefully evaluated and a decision was made to transition the operation of the South Campus Flow Cytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's invescytometry services were carefully evaluated and a decision was made to transition the operation of the South Campus Flow Cytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's invesCytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's invesCytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's investigators.

Shared resources include core facilities for cell culture, flow cytometry, imaging, microsurgery, physiology, proteomics, virus molecular biology, and yeast two hybrid screening.

The Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellCytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellcytometry and cell sorting.

Flow - cytometry and molecular diagnostic for optimizing diagnostic and follow - up of patients with hematologic diseases: collaboration with REL (Rete Ematologica Lombarda), European Leukemia Net and several other groups.

The facility is continuously expanding with the newest software and machinery for both sort and analysis capabilities of up to 18 fluorescent parameters, and is committed to meet all of the flow cytometry needs for the Hubrecht Institute, the UMC Utrecht and the Princess Máxima Center.

Committed to meet all of the flow cytometry needs for the Hubrecht Institute and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellcytometry needs for the Hubrecht Institute and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellCytometry Core facility offers state of the art instrumentation for routine flow cytometry and cellcytometry and cell sorting.

While mass cytometry offers a powerful and highly multiplexed method for profiling single cells, there are various technical considerations for researchers wanting to perform a mass cytometry assay for the first time, or at a more advanced level.

After editing, cells were analyzed for CD81 expression via flow cytometry.

Proteintech offers a variety of secondary antibodies suitable for Western Blotting, ELISA, cellular imaging, and flow cytometry.

Individual, edited (CD81 knockout) hiPS cells were expanded into clonal lines and analyzed for expression of CD81 and three pluripotency markers via flow cytometry using antibodies against CD81, Oct ‑ 4, TRA ‑ 1 ‑ 60, and SSEA - 4.

Flow Cytometry: This four day program is ideal for those who are looking for an in - depth, hands - on introduction to Flow Cytometry.

Flow cytometry (Fig. 4) revealed 57 % medium to large abnormal CD45 (dim +) cells within the nuclear cell gate with atypical cells positive for CD45 (slightly dim +), CD4 (dim +), CD13 -LRB--), CD15 -LRB--), CD33 (+), CD34 -LRB--), CD36 (partial +), CD38 (partial +), CD117 (variably +), HLADR (+), CD14 -LRB--), CD64 -LRB--), CD56 (+), and CD123 (+) which was compatible with BPDCN.

For details on how to make booking, please visit Cytometry confluence page.

Flow Cytometry Enables Multiplexed Measurements of Genetically Encoded Intramolecular FRET Sensors Suitable for Screening.

Someone who knows a thing or two about the importance of data collection is Michael Andreeff, M.D., Ph.D., professor of Leukemia, who was a pioneer in the develop of flow cytometry, a method for counting and sorting cells that is commonly used in today's clinical trials.

For a more indepth explanation, listing of services and common flow cytometry applications, click read more.

After editing, cells were analyzed for AcGFP1 expression and pluripotency via flow cytometry (Panel B) and immunocytochemistry (Panel C).

This material is intended for use as an internal flow cytometry control for detection of intracellular cytokines and validation of techniques.

A revolutionary cell processing technology enabling superior staining and retention of suspension cells for flow cytometry using centrifuge-less washing

After, cells were analyzed by flow cytometry or immunocytochemistry for AcGFP1 and pluripotency markers.

[B and E] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for leukocyte surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

Purified cells were surface stained for a variety of leukocyte markers and analyzed using flow cytometry.

In total, 106 splenocytes were cultured without peptide or with 0.2 μM LCMV gp33 peptide, NP396 peptide for 5 h. IFN - γ positivity by flow cytometry was used to identify LCMV - specific cells (13).

The current study involved monitoring the patients for one year after completed stem cell transplantation and used mass cytometry to study the different cell types of the immune system and the so called ProSeek method for simultaneous protein analysis.

(b) The same flow cytometry analysis for BCM2 (total cell population) and BCM2 F3 (cycling hypoxia - selected subpopulation) cells.

Overall, results of this evaluation qualify CytoFLEX LX as an easy - to - operate and very versatile instrument for several multiparameter flow cytometry applications, includinghigh content flow cytometry combined with absolute cell counting.

Phytoplankton ecology, photophysiology, bio-optical oceanography, modeling of marine primary production, physical forcing and regulation of phytoplankton biomass and production, flow cytometry, instrument development for autonomous phytoplankton observation, and remote sensing of phytoplankton.

Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.

[B] Brain leukocytes were percoll purified from the remaining brain and meningeal homogenate, stained for surface markers CD4, CD8, CD19, CD11b, and Ly6G, and assayed using flow cytometry.

Image cytometry of Cyclin D1: a prognostic marker for head and neck squamous cell carcinomas.

For confirming our study results, the correlation between Cyclin D1 - staining scores and the percentage of S - phase obtained by flow cytometry which showed no significant correlation between Cyclin D1 protein and increased S - phase %.

SciLifeLab's facility for mass - cytometry (CyTOF) is now part of the national infrastructure and thus available for applications by the entire Swedish research community.