Sentences with phrase «cytometry on»
Single cell Q - RTPCR for the genes indicated was carried out on cells separated by flow cytometry on the basis of cell surface marker expression.
http://journals.plos.org/
(C) Flow cytometry on cells plated at single cell density in 96 well plates from each sorted fraction.
GFP + leukemia cells in peripheral blood were counted by flow cytometry on day 7 (C) and day 14 (D).
Not exact matches
The centre will be structured around six technical platforms focusing on zebrafish transgenesis and micromanipulation, bioimaging, mouse transgenesis, flow cytometry, stem cell culture, and gene - expression analysis.
Her current research focuses on developing clinical applications for imaging flow cytometry, with special attention to transcription factor pathways.
He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.
They have screened combinations of antibodies that bind to specific proteins on the surface of the immature and mature stem cells and that can be used for flow cytometry, a common laboratory technique for sorting cells.
«We are working on similar principles,» he says, using particle image flow cytometry and high - speed cameras to try to reverse engineer a theoretical model.
«If you get cleavage, you'll lose the green tag,» said Cameron Smurthwaite, manager of SDSU's flow cytometry core facility and lead author on the Journal of Visualized Experiments paper.
Analytical flow cytometry analysis was performed on whole blood after lysis of erythrocytes and fixing in 2 % PFA.
IDMIT will contribute 1) To the development and validation of assays based on flow cytometry and mass cytometry for the evaluation of immune responses in humans and animal models; these tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal model platform, in particularly by providing access to NHP models and to new technologies for in vivo imaging infections and host responses; 3) To networking activities, in particular by organising a workshop on in vivo imaging.
Dr. Ruxandra Sîrbulescu analyzes flow cytometry data on immune cells used in a wound healing study.
Peter is also heavily involved with teaching microscopy and flow cytometry which includes organising and teaching on both the RMS Light Microscopy Summer School and the RMS Practical Flow Cytometrycytometry which includes organising and teaching on both the RMS Light Microscopy Summer School and the RMS Practical Flow CytometryCytometry courses.
I and a colleague have attempted to use this on two different types of cell lines using Flow cytometry and fluorescence microscopy all of which proved unsuccessful.
Our Luminex 100 and 200 systems are based on the principles of flow cytometry.
All flow cytometry data were acquired on an LSRII cytometer (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (Tree Star, Ashland, OR).
The expression of CCR5 on their surface was analyzed by laser flow cytometry.
Flow Cytometry: This four day program is ideal for those who are looking for an in - depth, hands - on introduction to Flow Cytometry.
For details on how to make booking, please visit Cytometry confluence page.
The Institute is currently running on a Site License by Flowjo, a flow cytometry data analysis software package which offers a range of analysis platforms that includes compensation, cell cycle, proliferation studies and kinetics.
Experts and Key Opinion Leaders on the topic will share their experiences and views on potency assays and cell characterization by flow cytometry.
O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer - Friedrich, J. Guck Real - time deformability cytometry: on - the - fly cell mechanical phenotyping.
The percentage of CSCs in the cycling hypoxia selected subpopulation was analyzed based on the CD44, CD24, ESA, and E-cadherin expression by three - color flow cytometry.
• Flowmetric — Founded by CEO, Ren Capocasale, Flowmetric is a contract research organization focused on providing polychromatic flow cytometry and cell sorting services to bio-pharma and drug discovery companies.
The two nodes perform standardized mass - cytometry experiments on customer provided samples using validated panels of metal - coupled antibodies.
Advanced training on MIC (Multiparametric Image Cytometry) will be dispensed at SFR BioSciences Gerland - Lyon Sud.
(C and D) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen of control mice and VPA recipients on the indicated days.
Conventional flow cytometry is a powerful technique for measuring cell phenotype and function, but it relies on fluorescent stains, or labels, to identify particular cell subpopulations.
The Gnoto / Axenic Facility of the Instituto Gulbenkian de Ciência hosted clients in previous user projects to perform on site e.g. histology, flow - cytometry or microscopy.
PHENOMIN - CIPHE, on the strength of its expertise in genetics and mouse immunology, is positioned as a pioneer in multiparametric analysis of the immune system of mutant mice in normal conditions or exposed to risks of infection, by an integration of innovative research and technical knowledge in the field of flow and mass cytometry as well as advanced microscopy.
(A and B) Flow cytometry analysis of intracellular IFN - γ and IL - 17A on donor - derived CD4 + T cells from spleen on indicated days for syngeneic and allogeneic mice.
Practical training on multi-parameter flow, mass and spectral cytometry (respectively MFC, MMC and MSC) will be performed on one single site at CIPHE (Marseille).
The purpose of the flow cytometry core facility is to sort cells for users, and to train facility users on the self - service cytometers and related software for data acquisition and analysis.
HV ES cells grown under self - renewing conditions were fractionated by flow cytometry into four fractions based on Venus (V) and SSEA - 1 (S) expression.
Flow cytometry is a powerful technique that allows researchers to examine multiple proteins on cell populations using fluorescently labeled antibodies.
Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single - cell suspensions based on differential expression of surface antigens.