Sentences with phrase «cytoplasmic fractions»
Anti-β-actin antibody (Sigma - Aldrich, St. Louis, MO, USA) was used to confirm equal protein loading of cytoplasmic fractions.
https://breast-cancer-research.biomedcentral.com/
Beta - actin is used as the loading control for the cytoplasmic fraction, and histone H3 is used as the loading control for the nuclear fraction.
Not exact matches
Basic annotation parameters include an evaluation of i) staining intensity (negative, weak, moderate or strong), ii) fraction of stained cells (rare, < 25 %, 25 - 75 % or > 75 %) and iii) subcellular localization (nuclear and / or cytoplasmic / membranous).