Sentences with phrase «cytosine methylation»

Furthermore, high - resolution analysis of cytosine methylation in primary and transformed cells has found less aberrant methylation of CpG island promoters in transformed cells than had been previously hypothesized based on candidate gene studies [58].

Our and previous findings suggest that both HDACs and cytosine methylation contribute to HIV - 1 latency, in agreement with a growing body of evidence demonstrating cooperation between these two gene silencing mechanisms [55], [56].

Citation: Kauder SE, Bosque A, Lindqvist A, Planelles V, Verdin E (2009) Epigenetic Regulation of HIV - 1 Latency by Cytosine Methylation.

Vlatka Zoldoš» lab has deposited pdCas9 - DNMT3A - EGFP and pdCas9 - DNMT3A - PuroR for targeted cytosine methylation in mammalian cells.

Cytosine methylation involves adding or removing methyl groups to cytosine, which is a building block of DNA.

The results suggest the mechanism called cytosine methylation plays a previously under - appreciated role in the development of leukemia.

The biological question that drives our research is how cytosine methylation represses transcription and ultimately impacts phenotypes.

Transformed cells such as the Jurkat line may show aberrant DNA methylation patterns at specific loci [57], possibly complicating analyses of cytosine methylation and HIV - 1 latency.

Importantly, we confirmed the association between HIV - 1 latency and cytosine methylation in a primary cell model of HIV - 1 latency.

Importantly, after each round of DNA replication, cytosine methylation is faithfully reproduced in a process that is directed by previously methylated DNA [54].

However, because J - Lat cells divide autonomously and possess other aberrations associated with cellular transformation, cytosine methylation was analyzed in a recently developed primary cell model of latency [43].