Sentences with phrase «days after transfection»
Lysates were harvested 3 days after transfection and subjected to immunoblotting.
https://elifesciences.org/
Several iPSC colonies were observed as early as 10 days after transfection, becoming morphologically indistinguishable from the original H1 - Oct4 - EGFP cell line (Fig. 6B, C).
B, Small iPSC colonies can be detected 10 days after transfection with pCEP - Oct4 and pCEP - Nanog.
Not exact matches
Supernatants were collected for 3 consecutive days beginning 48 hours after transfection.
Two days after infection / transfection, cells were plated on mitotically inactivated MEFs (Chemicon), with hESCs medium, in the presence or not of 50 µg / ml of hygromycin B (Invitrogen).
48 hr after transfection, cells were seeded into six - well plates in triplicates and selected with puromycin (1.5 μg / ml) for 3 days.