Sentences with phrase «de-greening gene circuit»

We have previously described a plant response system that produces white plants when a «de-greening gene circuit» is transcriptionally induced with an estrogen - like hormone [7].

Hence, we linked output of our complete synthetic sensing gene circuit to the de-greening gene circuit by placing its genes (diRNA POR, protochlorophyllide oxidoreductase; AtChlase, chlorophyllase; AtRCCR, red chlorophyll catabolite reductase)[7] under control of the PlantPho promoter (Figure 1).

Our previously described synthetic de-greening gene circuit shows a threshold - like response through generation of reactive oxygen species and photosystem disruption [7].

Response of transgenic tobacco plants with one copy of each gene circuit, de-greening gene circuit and synthetic sensing gene circuit.

The sensing gene circuit is contained on a T - DNA providing resistance to Basta whereas the de-greening gene circuit is on a T - DNA providing resistance to kanamycin.

We used quantitative RT - PCR to confirm and measure activation of the de-greening gene circuit (Figure 4D — E).

Because the response from the de-greening gene circuit involves threshold - like behavior, accuracy at lower levels of the ligand is currently more difficult to follow.

(D) Real - time qRT - PCR confirming that plants responding as shown in A, B, and C results from activation of the de-greening gene circuit.

Not exact matches

The line displaying the best response, NT4, contains multiple T - DNAs (Table S2) with segregation of the de-greening circuit genes (KanR) suggesting linkage.

Quantitative RT - PCR analysis of the de-greening circuit genes (Figure 4E) corroborates the weak response observed in the transgenic plants, with endogenous NtPOR mRNA levels decreasing to approximately one - third of the control levels, and AtRCCR increasing three-fold.

(E) Plants exposed to TNT vapor were analysed for induction of the de-greening circuit genes with Real - time qRT - PCR.